Antimicrobial Agents and Chemotherapy, April 2009, p. 1592-1597, Vol. 53, No. 4
Antimicrobial Activity of Curcumin against Helicobacter pylori Isolates from India and during Infections in Mice
Ronita De,1 Parag Kundu,2 Snehasikta Swarnakar,2 T. Ramamurthy,1 Abhijit Chowdhury,3 G. Balakrish Nair,1 and Asish K. Mukhopadhyay1*
National Institute of Cholera and Enteric Diseases, Kolkata 700010, India,1 Indian Institute of Chemical Biology, Kolkata 700032, India,2 School of Digestive Diseases, Institute of Post Graduate Medical Education and Research, Kolkata, India3
Treatment failure is a major cause of concern for the Helicobacter pylori-related gastroduodenal diseases like gastritis, peptic ulcer, and gastric cancer. Curcumin, diferuloylmethane from turmeric, has recently been shown to arrest H. pylori growth. The antibacterial activity of curcumin against 65 clinical isolates of H. pylori in vitro and during protection against H. pylori infection in vivo was examined. The MIC of curcumin ranges from 5 µg/ml to 50 µg/ml, showing its effectiveness in inhibiting H. pylori growth in vitro irrespective of the genetic makeup of the strains.
The nucleotide sequences of the aroE genes, encoding shikimate dehydrogenase, against which curcumin seems to act as a noncompetitive inhibitor, from H. pylori strains presenting differential curcumin MICs showed that curcumin-mediated growth inhibition of Indian H. pylori strains may not be always dependent on the shikimate pathway. The antimicrobial effect of curcumin in H. pylori-infected C57BL/6 mice and its efficacy in reducing the gastric damage due to infection were examined histologically.
Curcumin showed immense therapeutic potential against H. pylori infection as it was highly effective in eradication of H. pylori from infected mice as well as in restoration of H. pylori-induced gastric damage.
This study provides novel insights into the therapeutic effect of curcumin against H. pylori infection, suggesting its potential as an alternative therapy, and opens the way for further studies on identification of novel antimicrobial targets of curcumin.
Helicobacter pylori is a microaerophilic bacterium with the extraordinary ability to establish infections in human stomachs that can last for years or decades. It is carried by more than one-half of all people worldwide, and its prevalence exceeds 90% in some developing countries like India. It has attracted great attention as a major cause of peptic ulcer disease.
In fact, H. pylori is the first bacterium to be classified as a group I carcinogen by the International Agency for Research on Cancer (6, 31). Based on the genetic characteristics and disease outcome, there are significant geographic differences among H. pylori strains. Indian H. pylori strains are genetically distinct from those from east Asia and the West (4, 16). ...
Isn't turmeric a major spice used in Indian cuisine?