Doxycycline stops Prion Protein Infections

[Pandora]

Cycline efficacy on the propagation of human prions in primary cultured neurons is strain-specific.
http://www.ncbi.nlm.nih.gov/pubmed/24265435
J Infect Dis. 2013 Nov 21. [Epub ahead of print]

Hannaoui S, Gougerot A, Privat N, Levavasseur E, Bizat N, Hauw JJ, Brandel JP, Haïk S.
Source

Université Pierre et Marie Curie - Paris 6, Centre de Recherche de l'Institut du Cerveau et de la Moelle épinière (CRICM), UMRS 975, Equipe "Alzheimer's and Prion diseases", Paris, 75013, France.
Abstract

In prion diseases, a major issue in therapeutic research is the variability of the effect between strains. Stimulated by the report of an antiprion effect in a scrapie model and by ongoing international clinical trials using doxycycline, we studied the efficacy of cyclines against the propagation of human prions.

First, we successfully propagated various Creutzfeldt-Jakob disease isolates (sporadic, variant and iatrogenic CJD) in neuronal cultures

expressing the human prion protein.

Then, we found that doxycycline was the most effective compound, with important variations between isolates.

Isolates from sporadic CJD, the most common form of prion diseases showed the highest sensitivity.

[Pandora]

Prion, prionoids and infectious amyloid.
Parkinsonism Relat Disord. 2014 Jan;20S1:S80-S84. doi: 10.1016/S1353-8020(13)70021-X.
Liberski PP.
http://www.ncbi.nlm.nih.gov/pubmed/24262195
The proteins involved differ in each of these disorders

but the molecular mechanism is almost exactly the same, a seeding-nucleation mechanism.
------------
Aspirin helps too but you have to make sure your blood does not get too thin.
http://www.ncbi.nlm.nih.gov/pubmed/?term=aspirin+prion

From Prion Diseases to Prion-Like Propagation Mechanisms of Neurodegenerative Diseases
http://www.hindawi.com/journals/ijcb/2013/975832/

Video of Scientists Observe Infectious Prion Proteins Invade and Move Within Brain Cells
http://www.niaid.nih.gov/topics/prion/P ... video.aspx

Use of Nonelectrolytes Reveals the Channel Size and Oligomeric Constitution of the Borrelia burgdorferi P66 Porin
http://www.plosone.org/article/info%3Ad ... ne.0078272

[inmacdonald]

Doxycycline's Reach and Grasp Vistas - 2013 Re-Writes

Those educated in a time when Goodman and Gilman's Textbook of Pharmacology
Link:
http://www.amazon.com/s/ref=nb_sb_ss_i_ ... Caps%2C360

are now up against the ropes and being Pummeled by major changes in
the conventional wisdom of the Reach and Grasp of a pharmaceutical
which was taught to act by a Ribosomal Mechanism.

The above cited references challenge us to embrace new and heretofore
unimagined Pharmaceutical Mechanisms of Medicinal Chemistry in the human host.

Proteins - Pathologic merely because of Misfolding leads to 3 dimensional conformations..
-
Prions for which Pruisner campaigned for his own Nobel Prize with the promise
that such entities were Independent of DNA..

Did Dr. Stanley Pruisner know more than he was telling us in his acceptance speech
in Stockholm?

Is there Now
an "Explainer in Chief" to render the Realities of Doxycycline and DNA-independent Pathological Prions
to those of us here on the forum?
We need short Anglo Saxon Words free of Jargon to resolve these quandries...............
Who could fill the Bill??

Perhaps hv808ct, Resident Insider to the cognoscenti,
to the rescue?//????///???

Respectfully,
Alan B. MacDonald MD FCAP FASCP
November 24, 2013

[Pandora]

In awarding research that may otherwise go unnoticed, prize juries also have an important role in sending a message to the scientific community about the importance of a particular research area.

Stanley Prusiner's discovery that prions are the causal agent of scrapie, mad cow disease and Creutzfeldt–Jakob disease was not widely accepted until he won the Lasker Award and the Nobel Prize for his work.

Robin Warren and Barry Marshall's discovery that Helicobacter pylori cause gastritis and stomach ulcers was largely ignored by the medical community,

who could not accept that a simple bacterium could be the cause of peptic ulcers.

However, the fact that Marshall won a Lasker and a John Scott Award in 1995 and a Gairdner Foundation International Award in 1996, and shared the 1994 Warren Alpert Prize and the 1997 Ehrlich Prize with Warren, helped to make their discovery more acceptable. “It was a good idea and moderate methodology, but the perspective behind it is enormous,” explained Fleckenstein.

“Sometimes, you want to show a bit of courage.”

Jury members also showed courage in awarding the 2005 Ehrlich Prize, worth euro100,000, to Ian Wilmut for his cloning experiments, a decision that was vehemently criticized in Germany by opponents of cloning and stem-cell research.

...prize juries have an important role in sending a message to the scientific community about the importance of a particular research area
http://www.nature.com/embor/journal/v6/ ... 00523.html
-------------------
COURAGE!!!

[inmacdonald]

I like the word "courage"

synomym
GONADAL Fortitude !

"The Emperor's New Clothes" reincarnated each day by those who fear to speak plainly

Thanks so much Pandora!!!
Best,
Alan

[lou]

Not everyone thinks prions are causative. But rather a result of a stealth bacterial infection. So Nobel or not, this could explain why doxy works.

[Pandora]

Yes lou. Spirochetes have at least 3 or 4 proteins described as ""prion- like"" found so far. Their recombinations in theory are infinite.
Their recombinations mostly reside in the skin.
http://www.lymeneteurope.org/forum/view ... 246#p36246

[Pandora]

A BASTION OF COURAGE WE HAVE FOUND!

The case for involvement of spiroplasma in the pathogenesis of transmissible spongiform encephalopathies.

http://www.ncbi.nlm.nih.gov/pubmed/24423635
J Neuropathol Exp Neurol. 2014 Feb;73(2):104-14. doi: 10.1097/NEN.0000000000000033.

Bastian FO.
Author information
Abstract
Spiroplasma biofilm formation explains the role of these wall-less bacteria in the pathogenesis of transmissible spongiform encephalopathies (TSEs). Spiroplasma embedded in the biofilm polysaccharide matrix are markedly resistant to physical and chemical treatment, simulating the biologic properties of the TSE agent. Microcolonies of spiroplasma embedded in biofilm bound to clay are the likely mechanism of lateral transmission of scrapie in sheep and chronic wasting disease in deer via soil ingestion. Spiroplasma in biofilm bound to the stainless steel of surgical instruments may also cause iatrogenic transmission of Creutzfeldt-Jakob disease. Sessile spiroplasma in biofilm attach to the surface by curli-like fibrils, a functional amyloid that is important for spiroplasma entering cells.

Curli fibers have been shown to interact with host proteins and initiate formation of a potentially toxic amyloid that multiplies by self-assembly.

In TSE, this mechanism may explain how spiroplasma trigger the formation of prion amyloid. This possibility is supported by experiments that show spiroplasma produce α-synuclein in mammalian tissue cultures. The data linking spiroplasma to neurodegenerative diseases provide a rationale for developing diagnostic tests for TSE based on the presence of spiroplasma-specific proteins or nucleic acid. Research efforts should focus on this bacterium for development of therapeutic regimens for Creutzfeldt-Jakob disease.
--------------------------
http://www.ncbi.nlm.nih.gov/pubmed/24411709
No Doxy won't cure all the woes because in the immune suppression from the REAL cause of AIDS Spirochetes are not the only infections they have.....

They have multiple chronic infections in ALL syndromes.
http://www.youtube.com/watch?v=yOno_2m_8LY

Could the combination of Doxy with other do the job? No it will not stop the immune suppression. For that they need their stem cells after killing all they can to raise the gates of the immune systems the stealth has destroyed.

HOPE HE STAYS OUT OF MEMPHIS....LymeFLU EXPERT DON WILEY WASN'T SO LUCKY.
http://www.youtube.com/watch?v=H29IBRlFn-M
http://en.wikipedia.org/wiki/File:Elect ... A_H7N9.png
http://molecularalzheimer.org/files/Cel ... tic_Bb.pdf

[Pandora]

Mol Microbiol. 2014 Jan 14. doi: 10.1111/mmi.12518. [Epub ahead of print]
Direct assessment in bacteria of prionoid propagation and phenotype selection by Hsp70 chaperone.
http://www.ncbi.nlm.nih.gov/pubmed/24417419
Gasset-Rosa F, Coquel AS, Moreno-Del Álamo M, Chen P, Song X, Serrano AM, Fernández-Tresguerres ME, Moreno-Díaz de la Espina S, Lindner AB, Giraldo R.
Author information

Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas - CSIC, C/ Ramiro de Maeztu 9, E-28040 Madrid, Spain.

Abstract

Protein amyloid aggregates epigenetically determine either advantageous or proteinopathic phenotypes. Prions are infectious amyloidogenic proteins, whereas prionoids lack infectivity but spread from mother to daughter cells.

While prion amyloidosis has been studied in yeast and mammalian cells models, the dynamics of transmission of an amyloid proteinopathy has not been addressed yet in bacteria.

Using time-lapse microscopy and a microfluidic set-up, we have assessed in Escherichia coli the vertical transmission of the amyloidosis caused by the synthetic bacterial model prionoid RepA-WH1 at single cell resolution within their lineage context.

We identify in vivo the co-existence of two strain-like types of amyloid aggregates

within a genetically identical population and a controlled homogeneous environment.

The amyloids are either toxic globular particles or single comet-shaped aggregates that split during cytokinesis and exhibit milder toxicity. Both segregate and propagate in sub-lineages, yet show interconversion. ClpB (Hsp104) chaperone, key for spreading of yeast prions, has no effect on the dynamics of the two RepA-WH1 aggregates.

However, the propagation of the comet-like species is DnaK (Hsp70)-dependent. The bacterial RepA-WH1 prionoid thus provides key qualitative and quantitative clues on the biology of intracellular amyloid proteinopathies.
-----------------------
You see Lyme was never just about Spirochetes.....

[Pandora]

25 December
MERRY CHRISTMAS- JAPAN TRYING TO SAVE YOU...
Insect Cell-Derived Cofactors Become Fully Functional after Proteinase K and Heat Treatment for High-Fidelity Amplification of Glycosylphosphatidylinositol-Anchored Recombinant Scrapie and BSE Prion Proteins.
http://www.ncbi.nlm.nih.gov/pubmed/24367521
PLoS One. 2013 Dec 18;8(12):e82538. doi: 10.1371/journal.pone.0082538.

They used Insect Adjuvant in Flu vaccine. But I suspect they used them in food colorings, and GMO much earlier as evidenced in Morgellon sufferers gross recombinations exiting the skin that is easily produced with animal wormer paste and micro magnification-except in those most severely immune compromised. Any form of mild acid works as well. A drop of Lemon juice all over hands or feet.
They may require a doxy challenge to get them to the outer layers of skin where they can be induced to exit.
http://www.morgellons-research.org/morg ... oscop3.htm