Reactividad humoral contra Borrelia burgdorferi en pacientes con enfermedades dérmicas y reumáticas.
[Humoral reactivity against Borrelia burgdorferi in patients with skin and rheumatic diseases.]
Acta Med Colomb 20, 1995: 257-261.
http://www.actamedicacolombiana.com/ane ... rferi.html [free full text, Spanish]Summary
Lyme disease (LD) is the most studied infectious disease in the United States of America (USA) second only to the acquired immunodeficiency Syndrome. There is only one report in Colombia of several cases of rheumatological diseases with a positive serological reactivity to Borrelia burgdorferi. With the purpose of gaining additional information of this disease in Colombia, we carried out a prospective study in individuals with rheumatological and dermatological disease suggestive of LD according to the criteria of the Center for Disease Control in United States.
A total of 31 patients were included in the study, 23 had dermatological diseases and 8 had rheumatological diseases. Immunoglobulin G humoral reactivity against 3 B burgdorferi external membrane proteins (pl00, p41 and p41/l) was determined. Anti-cardiolipin antibodies were determined in all patients by means of the VDRL test. The Warthin-Starry stain was performed in skin biopsies from 19 patients with dermatological diseases in order to identifyt he presence of the spirochete. Only one patient with a clinical diagnosis of localized morphea had a positive serological reactivity to B burgdorferi (reactivity to all 3 membrane proteins), non-reactive VDRL, negative Warthin- Starry stain, and no clinical history of tick bites. In other 7 patients with localized morphea and in 3 with rheumatological diseases the serological reactivity was suggestive (weak or incomplete reactivity). The results of this study suggest the existence of LD in Colombia. Additional studies are required to isolate the microorganism from patients as well as vectors and to define the eco-epidemiological characteristics of the disease in Colombia.
J Med Entomol. 1998 May;35(3):324-6.
Searching for Lyme disease in Colombia: a preliminary study on the vector.
Mattar S, Lopez Valencia G.
Faculty of Sciences, Department of Microbiology, University of Javeriana, Bogota D.C., Colombia.
Lyme disease is an infectious multisystemic illness with dermatologic, neurologic, cardiac, and rheumatic manifestations. A total of 4,355 ticks was collected in Colombia, of which 2,805 were identified as Ixodes spp. The midgut contents of 2,600 specimens were fixed on microscope slides and examined by an indirect immunofluorescence assay (IFA) using monoclonal antibodies, anti-ospA H5332, and anti-flagellin 9724. All of the ticks examined by IFA tested negative.
Mem Inst Oswaldo Cruz. 1999 Jul-Aug;94(4):499-503.
Positive IgG Western blot for Borrelia burgdorferi in Colombia.
Palacios R, Osorio LE, Giraldo LE, Torres AJ, Philipp MT, Ochoa MT.
Centro Internacional de Entrenamiento e Investigaciones Médicas (Cideim), Cali, Colombia.
In order to evaluate the presence of specific IgG antibodies to Borrelia burgdorferi in patients with clinical manifestations associated with Lyme borreliosis in Cali, Colombia, 20 serum samples from patients with dermatologic signs, one cerebrospinal fluid (CSF) sample from a patient with chronic neurologic and arthritic manifestations, and twelve serum samples from individuals without clinical signs associated with Lyme borreliosis were analyzed by IgG Western blot. The results were interpreted following the recommendations of the Centers for Diseases Control and Prevention (CDC) for IgG Western blots. Four samples fulfilled the CDC criteria: two serum specimens from patients with morphea (localized scleroderma), the CSF from the patient with neurologic and arthritic manifestations, and one of the controls. Interpretation of positive serology for Lyme disease in non-endemic countries must be cautious. However these results suggest that the putative "Lyme-like" disease may correlate with positivity on Western blots, thus raising the possibility that a spirochete genospecies distinct from B. burgdorferi sensu stricto, or a Borrelia species other than B. burgdorferi sensu lato is the causative agent. Future work will focus on a survey of the local tick and rodent population for evidence of spirochete species that could be incriminated as the etiologic agent.
http://www.scielo.br/scielo.php?script= ... en&nrm=iso [free full text]
http://www.scielo.br/pdf/mioc/v94n4/3699.pdf [free full text, pdf]