Differentation of Borrelia microbes

Topics with information and discussion about published studies related to Lyme disease and other tick-borne diseases.
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Margherita
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Joined: Thu 27 Sep 2012 18:22

Differentation of Borrelia microbes

Post by Margherita » Wed 27 May 2015 16:05

Differentiation of Borrelia Microbes from Collagen Debris and Collagen Fibrils in Blood Cultures



Abstract

Lyme disease in humans is caused by Borrelia burgdorferi sensu lato, organisms that
are known to attach to collagen in the body. The use of a matrix containing collagen
as a part of the culture composition has given us the ability to cultivate Borrelia
burgdorferi, B. afzelii, and B. garinii from the bloodstream using artificial media. The
cultured Borrelia is identified by immunostaining using polyclonal and monoclonal
antibodies. In order to distinctly differentiate the organisms from the collagen of this
matrix that could be observed as background in the staining process, we developed
an immunostaining procedure using polyclonal and monoclonal antibodies in
combination with rhodamine fibronectin. The culture samples from both control
organisms and patient samples were tested using the new immunostaining protocol.
Results showed clear delineation of organisms compared to the collagen pieces
gathered in the harvesting process. This new immunostaining process, used with in
vitro cultivation, provides for precise identification of cultured organisms.
Source: Journal of Microbiology & Experimentation - Vol. 1 - Issue 2 2015
Authors: Sheila Wood*, Akshita Datar, Namrata Pabbat, Divya Burugu and Amy Rattelle
Advances Laboratories Services- Rowan University USA


For the full article: http://www.advanced-lab.com/news/RF_Publication.pdf

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