Lyme disease caused by Borrelia burgdorferi with two homeologous 16S rRNA genes: a case report

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ChronicLyme19
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Lyme disease caused by Borrelia burgdorferi with two homeologous 16S rRNA genes: a case report

Post by ChronicLyme19 » Fri 22 Apr 2016 14:14

https://www.dovepress.com/lyme-disease- ... icle-IMCRJ

Lyme disease caused by Borrelia burgdorferi with two homeologous 16S rRNA genes: a case report
Sin Hang Lee,1,2
1Pathology Department, Milford Hospital, Milford, CT, USA; 2Milford Molecular Diagnostics, Milford, CT, USA

International Medical Case Reports Journal
Received 5 November 2015
Accepted for publication 28 January 2016
Published 21 April 2016 Volume 2016:9 Pages 101—106
DOI http://dx.doi.org/10.2147/IMCRJ.S99936
Abstract: Lyme disease (LD), the most common tick-borne disease in North America, is believed to be caused exclusively by Borrelia burgdorferi sensu stricto and is usually diagnosed by clinical evaluation and serologic assays. As reported previously in a peer-reviewed article, a 13-year-old boy living in the Northeast of the USA was initially diagnosed with LD based on evaluation of his clinical presentations and on serologic test results. The patient was treated with a course of oral doxycycline for 28 days, and the symptoms resolved. A year later, the boy developed a series of unusual symptoms and did not attend school for 1 year. A LD specialist reviewed the case and found the serologic test band patterns nondiagnostic of LD. The boy was admitted to a psychiatric hospital. After discharge from the psychiatric hospital, a polymerase chain reaction test performed in a winter month when the boy was 16 years old showed a low density of B. burgdorferi sensu lato in the blood of the patient, confirmed by partial 16S rRNA (ribosomal RNA) gene sequencing. Subsequent DNA sequencing analysis presented in this report demonstrated that the spirochete isolate was a novel strain of B. burgdorferi with two homeologous 16S rRNA genes, which has never been reported in the world literature. This case report shows that direct DNA sequencing is a valuable tool for reliable molecular diagnosis of Lyme and related borrelioses, as well as for studies of the diversity of the causative agents of LD because LD patients infected by a rare or novel borrelial variant may produce an antibody pattern that can be different from the pattern characteristic of an infection caused by a typical B. burgdorferi sensu stricto strain.
Half of what you are taught is incorrect, but which half? What if there's another half missing?

Lorima
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Re: Lyme disease caused by Borrelia burgdorferi with two homeologous 16S rRNA genes: a case report

Post by Lorima » Tue 26 Apr 2016 2:22

"...LD patients infected by a rare or novel borrelial variant may produce an antibody pattern that can be different from the pattern characteristic of an infection caused by a typical B. burgdorferi sensu stricto strain."

<snort> As if we know what pattern, or strain, is "characteristic" or "typical."

Still, I'm glad it was published, and I know medical authors have to toe the IDSA line even if by some chance they know better.
"I have to understand the world, you see."
Richard Feynman

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inmacdonald
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Re: Lyme disease caused by Borrelia burgdorferi with two homeologous 16S rRNA genes: a case reporthas

Post by inmacdonald » Wed 4 May 2016 15:38

God created microbes with one 16s rDNA per chromosome - at least this is the CONVENTIONAL WISDOM. CONVENTIONAL WISDOMS ARE OVERTURNED BY SCIENTIFIC DISCOVERIES, HOWEVER.

Dr Lee uses a proprietary PCR Which targets A portion of the 16s rDNA of Borrelia burgdorferi.
His test is available to the public for $150.00 per analyate

Since each Borrelia spirochete follows the rules, i.e. PERHAPS ONLY ONE 16s per chromosome,
The notion of a "doublet" of 16s rDNA SITES MUST BE RECONCILED WITH THE RULES OF MOLECULAR MICROBIOLOGY.

1. There is at present no published precedent for. "Doublet " of. 16srDna residing in a single microbe.
2. No one has investigated any microbe for detection of simultaneous multiple 16s rDNA open reading frames
3. The number of possible chromosomes per Borrelia spirochetes varies from 15 chromosomes to over 50 chromosomes per single Borrelia spirochete. These numbers have been defined by
Dr. Alan G. Barbour , originally for Relapsing Fever Borrelia ( chromosome complement = 16 per Borrelia cell). Subsequent studies by Barbour in Burgdorferi sl spirochetes yielded
Chromosome complement per burgdorferi spirochete = >16 to > 50.
4. Each individual chromosome in a Borrelia cell is autonomous, and is derived from a pre-existing single chromosome. It is then allowed that each Borrelia cell has DIVERSITY I the DNA makeup of Each Of ITS 16 chromosomes TO 50 chromosomes.
5. Barbour has published reports of CHIMERAS at the PLASMID LEVEL for Relapsing Fever Borrelia. Barbour has not researched any Borrelia species ( RFB OR BURGDORFERI (sl) )
For detection of CHROMOSOME CHIMERAS.
6. MacDonald has identified CHIMERAS IN Burgdorferi Borrelia by Fluorescence in Situ DNA hybridization studies. He used the Approach of Barbour, namely simultaneous Dna Two color Probe hybridization . Red probe alone produces a Red signal. Green probe alone produces a Green signal.
Simultaneous Red probe binding and Green Probe binding Within a single Borrelia cell yields the Chimeric Hybridization Product signal ( Red plus Green Equals YELLOW Signal in an individual spirochete)
7. Dr. Lee has identified In a mixture of Borrelia DNA extracts from One patient
A "doublet Signal ". ( two unique 16s DNA Target sites)
8. My conclusion : Rule out 16s diversity in two separate borrelia cells. VERSUS
Rule out 16s CHIMERAS In a novel unknown Borrelia species - with all
Chimeras identical to each other
Rule out pre existing 16s Diversities within a single population of
Burgdorfer Borrelia ( i.e. The chromosomes among the 16 per
Borrelia cell. Are not 16s identical ,,.. And this could include the
STRAIN B31 progeny
The notion of COOKIE CUTTER population LYME Spirochetes is a false concept.

9. Serodiagnosis , Flawed in 1982, Flawed today, should be abandoned.
The Best pathway to Abandonment of Serodagnosis is to WITHHOLD HEALTH INSURANCE PAYMENT FOR ALL LYME ELISA AND ALL LYME WESTERN BLOTS.

RESPECTFULLY

Alan B. MacDonald,MD, FACP
May 4, 2016

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