Apoptosis....fibroblasts infected with Bbsl

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lou
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Apoptosis....fibroblasts infected with Bbsl

Post by lou » Sat 25 Mar 2017 14:20

Induction of Apoptosis in Normal Human Dermal Fibroblasts Infected with Borrelia burgdorferi Sensu Lato

To cite this article:
Rozwadowska Beata, Albertyńska Marta, Okła Hubert, Jasik Krzysztof P., Swinarew Andrzej S., Mazurek Urszula, Dudek Sławomir, Urbańska-Jasik Danuta, and Poprawa Izabela. Vector-Borne and Zoonotic Diseases. April 2017, 17(4): 237-242. doi:10.1089/vbz.2016.2057.

Published in Volume: 17 Issue 4: April 1, 2017
Online Ahead of Print: January 9, 2017
Full Text HTML Full Text PDF (336.9 KB) Full Text PDF with Links (196.7 KB)

Author information
Beata Rozwadowska,1 Marta Albertyńska,1 Hubert Okła,1,2 Krzysztof P. Jasik,1 Andrzej S. Swinarew,2 Urszula Mazurek,3 Sławomir Dudek,4 Danuta Urbańska-Jasik,5 and Izabela Poprawa5
1Department of Skin Structural Studies, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice, Poland.
2Institute of Materials Science, Faculty of Computer Science and Materials Science, University of Silesia in Katowice, Poland.
3Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice, Poland.
4Department of Pharmacognosy and Phytochemistry, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice, Poland.
5Department of Animal Histology and Embryology, Faculty of Biology and Environmental Protection, University of Silesia in Katowice, Poland.
Address correspondence to:
Beata Rozwadowska
Department of Skin Structural Studies
School of Pharmacy with Division of Laboratory Medicine
Medical University of Silesia in Katowice
ul. Kasztanowa 3
Sosnowiec, 41-200
Poland
E-mail: brozwadowska@op.pl
Krzysztof P. Jasik
Department of Skin Structural Studies
School of Pharmacy with Division of Laboratory Medicine
Medical University of Silesia in Katowice
ul. Kasztanowa 3
Sosnowiec, 41-200
Poland


ABSTRACT
The spirochete Borrelia burgdorferi s.l. can enter into different eukaryotic cells. Intracellular localization of bacteria may cause many changes in different cell pathways like apoptosis-mediated caspase cascade. The present studies focused on gene expression associated with caspase cascade after normal human dermal fibroblasts (NHDF) infection with Borrelia garinii, Borrelia afzelii, and B. burgdorferi s.s. The use of oligonucleotide microarray technique enabled an expression level comparison of genes associated with caspase cascade in NHDF infected with spirochetes. The increased expression of genes associated with caspase cascade was observed in case of CASP5, CASP2, CARD10, CASP10, MALT1, and NLRP1. The decreased expression was observed in case of CASP4, CASP6, and CASP1. The mRNA expression for CASP3 was inhibited in cells infected with three genospecies of Borrelia. However, the intensity of fluorescence was not statistically significant. In addition, cell cultures were fixed and procedure of caspase-3 detection and the TUNEL assay were performed. The in situ caspase-3 detection procedure confirmed the results obtained from microarray analyses. Only several fluorescent signals were observed. Many apoptotic cells were detected in NHDF-infected cultures with all spirochete genospecies found using the TUNEL reaction.

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