Page 1 of 8

Improved Borrelia burgdorferi Blood culture Method

Posted: Mon 18 Feb 2013 16:05
by inmacdonald
Link:
http://www.medsci.org/v10p0362.htm

Int J Med Sci 2013; 10(4):362-376. doi:10.7150/ijms.5698

Research Paper

Improved Culture Conditions for the Growth and Detection of Borrelia from Human Serum

Eva Sapi1,2 , Namrata Pabbati1, Akshita Datar1, Ellen M Davies1, Amy Rattelle1, Bruce A Kuo1

1. Research Division of Advanced Laboratory Services Philadelphia PA, USA;
2. Department of Biology and Environmental Science, University of New Haven, West Haven CT, USA.

_______________________________________
Alan MacDonald MD : Comment:
This paper provides Blood culture evidence backed up by DNA sequencing of the individual isolates
of Borrelia burgdorferi .All patients are those with Chronic Persistent Lyme Borreliosis
who have undergone long term antibiotic therapy and who
were off any antibiotics for a minimum of 6 week prior to the procurement of the blood cultures

The Gold Standard for Diagnosis of Lyme Borreliosis as per CDC guidelines is satisfied in all of the
cases presented here.
The IDSA dogma that no Chronic Lyme Disease exists is overturned by the blood cultures in their series of
patients.

The implications for Treatment are clear.
The implications for Diagnosis are Clear.

The only unknown from this point forward is the exact moment when the IDSA will issue
a formal apology to all of those patients who, have endured years of the medical establishmnet denials, health
insurance company coverage abrogations, and political recriminations for speaking plainly about the
chronicity of their Illness,
____________________________
Respectfully submitted,
Alan B, Macdonald MD FCAP,FASCP
Feburary 18, 2013

Re: Improved Borrelia burgdorferi Blood culture Method

Posted: Mon 18 Feb 2013 16:21
by Bagge
.
inmacdonald wrote:Link:
http://www.medsci.org/v10p0362.htm

Int J Med Sci 2013; 10(4):362-376. doi:10.7150/ijms.5698

Research Paper

Improved Culture Conditions for the Growth and Detection of Borrelia from Human Serum

Eva Sapi1,2 , Namrata Pabbati1, Akshita Datar1, Ellen M Davies1, Amy Rattelle1, Bruce A Kuo1

1. Research Division of Advanced Laboratory Services Philadelphia PA, USA;
2. Department of Biology and Environmental Science, University of New Haven, West Haven CT, USA.

_______________________________________
Alan MacDonald MD : Comment:
This paper provides Blood culture evidence backed up by DNA sequencing of the individual isolates
of Borrelia burgdorferi .All patients are those with Chronic Persistent Lyme Borreliosis
who have undergone long term antibiotic therapy and who
were off any antibiotics for a minimum of 6 week prior to the procurement of the blood cultures

The Gold [sic]Standard[sic] for Diagnosis[sic] of Lyme Borreliosis as per CDC guidelines is satisfied in all of the
cases presented here.
The IDSA dogma that no Chronic [sic]Lyme Disease [sic]exists is overturned by the blood cultures in their series of
patients.

The implications for Treatment[sic] are clear.
The implications for Diagnosis[sic] are Clear[sic].

The only unknown from this point forward is the exact moment when the IDSA will issue
a formal apology to all of those patients who, have endured years of the medical establishmnet[sic] denials, health
insurance company coverage abrogations, and political recriminations for speaking plainly about the
chronicity of their Illness,
____________________________
Respectfully submitted,
Alan B,[sic] Macdonald [sic]MD FCAP,[sic]FASCP
Feburary [sic]18, 2013

Continually starting new threads about the same test does not make it's history or questionable integrity disappear.

Information about this test can be found at the following links.

http://www.lymeneteurope.org/forum/view ... 0&start=70

http://www.lymeneteurope.org/forum/view ... f=7&t=3828

http://www.lymeneteurope.org/forum/view ... =20#p33910

Medscape registration required: http://www.medscape.com/viewarticle/778482

http://relative-risk.blogspot.com/2013/ ... itive.html

http://relative-risk.blogspot.com/2012/ ... sease.html
.

Re: Improved Borrelia burgdorferi Blood culture Method

Posted: Mon 18 Feb 2013 16:32
by inmacdonald

For the Reading ImPaired:

NOTICE:


THIS IS THE PEER REVIEWED PUBLISHED MANUSCRIPT
and not a prepublication posting.



All or the Data in the Final Published Document
has never appeared before [ pre-publication Embargo policy - standard for Peer Reviewed mnnuscripts}

Re: Improved Borrelia burgdorferi Blood culture Method

Posted: Mon 18 Feb 2013 16:43
by Bagge
inmacdonald wrote:
For the Reading[sic] ImPaired[sic]:

NOTICE:


THIS IS THE PEER REVIEWED PUBLISHED MANUSCRIPT
and not a prepublication posting.



All or[sic] the Data[sic] in the Final [sic]Published [sic]Document[sic]
has[sic] never appeared before [ pre-publication Embargo policy - standard for Peer Reviewed mnnuscripts}[sic]
As I understand, the research of this product was peer-reviewed as well.

http://www.fda.gov/ICECI/EnforcementAct ... 193174.htm
• "Germ Slayer killed 100% of the Borrelia bacteria without allowing it to convert to the cyst stage. This is a significant finding in the fight against Lyme disease."
http://www.lymeneteurope.org/forum/view ... =40#p34315
.

Re: Improved Borrelia burgdorferi Blood culture Method

Posted: Mon 18 Feb 2013 20:12
by hv808ct
MacDonald:
For the Reading ImPaired:
NOTICE:
THIS IS THE PEER REVIEWED PUBLISHED MANUSCRIPT
and not a prepublication posting.
Well, well. Another magical system for culturing phantom borrelia from the chronically infected. I don’t suppose we can expect to hear any presentations by Sapi et al. at ASM or ICAAC this year? Probably not. Likely there would be too many questions about the origins of the patient samples and how those patients were determined to have had Lyme disease. Still, this open source publication should encourage a couple of competent investigators to try to reproduce the alleged results. I suspect the end result will be pretty much what happened with the infamous Phillips culture system back in 2000 (http://www.ncbi.nlm.nih.gov/pubmed/11060098). Total bust.

From my perspective, the most interesting part of this paper is in the Acknowledgements.

The authors thank Dr. Joseph Burrascano, Jr., Dr. Alan MacDonald and Dr. Parkash Gill for helpful discussion.
This work was supported by a grant from RAM Capital LLC to the Research Division of Advanced Laboratory Services Inc (ALSI). During this study NP, AD, EAM, BAK were employees of ALSI and ES was a consultant. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors have declared that no competing interests exist.

Burrascano, the mysterious RAM Capital investment company and Adv. Lab. A curious mix. Interesting that everyone got paid by Adv. Lab. Frankly, the paper seems like little more than an advertisement for the very expensive ALS diagnostic test, and as a published excuse for Lyme quacks to continue pumping expensive antibiotics and supplements into gullible Lyme patient wannabes.

Re: Improved Borrelia burgdorferi Blood culture Method

Posted: Mon 18 Feb 2013 20:58
by duncan
"...Lyme patient wannabes."

:shock:

Curious observation. Who aspires to be a Lyme patient?? Interesting company one must keep to so aspire, I'd wager. Leper colony, maybe?

Re: Improved Borrelia burgdorferi Blood culture Method

Posted: Mon 18 Feb 2013 22:52
by tosho
From a patient perspective, I would like to see this test confirmed by independent researchers, who are not on the stage of TBDs controversy. I don't like the way this test has been introduced to people, however I keep fingers crossed that the research will turn out to be solid (as with any research that may improve TBDs patients' situations).

Re: Improved Borrelia burgdorferi Blood culture Method

Posted: Tue 19 Feb 2013 2:36
by LHCTom
These people are dishonest scientists who don't want to know the truth.

Wormser was able to achieve 40% sensitivity of serum culture.

For those who do not trust ALS, just use Wormser's formula.

Even at 40%, 4/10 of the patients would get a definitive diagnosis of an ongoing infection, something other tests cannot do.

Why would all these cruel people deny help to ill people?

The reason commonly stated for culture not being used in a clinical setting is cost, labor and the delay.

But if one is ill and is willing to pay for the cost and labor and wait the time for a 40% probability of a definitive answer, why not?

Because so many careers are tied up with the denial side, any culture would open up the truth.

From "Borrelia Molecular Biology Host Interactions and Pathogenesis"
The rate of recovery of B. burgdorferi s.s. from blood or blood components of untreated patients with erythema migrans had generally been 5% or less (Benach et al., 1983; Goodman et al., 1995; Steere et al., 1983b; Wallach et al., 1993), and until recently this source of culture material was largely abandoned. In past studies on the sensitiv¬ity of blood cultures for recovery of B. burgdorferi s.s., only a very small volume of blood or blood components (usually less than 1 ml) was cultured (Nadelman et al., 1990; Benach et al., 1983; Goodman et al., 1995; Steere et al., 1984; Steere et al., 1983b; Wallach et al., 1993; Nadelman et al., 1994). In view of the recommendation to culture quantities of blood as large as 20-30 ml in other bacterial infections, the rationale of culturing much smaller volumes for Lyme borreliosis pa¬tients was open to challenge. In a series of experi¬ments in adult patients with erythema migrans from the USA, recovery of B. burgdorferi s.s. was better from serum than from an identical volume of whole blood (Wormser et al., 1998), and the yield from plasma was significantly greater than that from serum (Wormser et al., 2000a). Yield directly correlated with the volume of material cultured (Wormser et al., 2001; Wormser et al., 2000a; Coulter et al., 2005). Recovery of B. burg¬dorferi s.s. from high-volume cultures of 9 ml of plasma inoculated into a modified BSK II prepa¬ration devoid of antimicrobials and gelatin, with a 20:1 ratio of medium to plasma, was consistently above 40% (Wormser et al., 2001; Wormser et al., 2000a). Unfortunately, high volume plasma cultures are not appropriate for young children since obtaining such a large quantity of blood would be unacceptable. Reported recovery rates from blood of patients with erythema migrans in Europe have been < 10% (Arnez et al., 2001; Maraspin et al., 2001). This may be due to a low frequency of haematogenous dissemination of B. afzelii, the principal cause of erythema migrans in Europe (Wang et al., 1999), or to culturing an inadequate volume of blood.
For several reasons, however, culture has not been used for diagnostic purposes in routine clini¬cal practice. There has been the lack of consistent availability of high-quality (i.e. borrelial growth promoting) lots of liquid medium for growing B. burgdorferi. Furthermore, by most conventional bacteriological standards, borrelial cultures are more labour intensive, expensive, and much slower, requiring up to 12 weeks of incubation before being considered negative.
But what about positives? So much for the Hippocratic Oath!

Re: Improved Borrelia burgdorferi Blood culture Method

Posted: Tue 19 Feb 2013 20:33
by nnecker
Burrascano: Advanced Research Corporation

http://investing.businessweek.com/resea ... vanced%20R

Mirra: Advanced Research Corporation

http://www.corporationwiki.com/Pennsylv ... 95570.aspx

Mirra: Ram Capital

http://sec.edgar-online.com/allion-heal ... ion13.aspx


Promissory Notes Held by and Transition Services Agreement with RAM Capital Group, LLC. Also in connection with the acquisition of Biomed on April 4, 2008, we assumed indebtedness of Biomed, which is payable to RAM Capital Group, LLC, or RAM Capital, pursuant to two promissory notes executed in favor of RAM Capital. In addition, we entered into a transition services agreement with RAM Capital immediately following the acquisition of Biomed. MR. MIRRA, A RELATED PERSON, IS THE SOLE OWNER OF RAM CAPITAL and therefore has an interest in the full value of the two promissory notes and the transition services agreement described below.

Mirra: Advanced Laboratory Services

http://www.corporationwiki.com/Pennsylv ... 91973.aspx

Mirra: Allion Health Care

http://www.ftc.gov/os/fedreg/2008/may/0 ... lyterm.pdf

http://www.gigijordanbail.com/press/Loc ... ST.com.pdf

Mirra: Ambulatory Pharmaceutical Services. pg .72

http://www.gigijordanbail.com/files/exhibits/QQ.pdf

Quote from Henry,a trained bacteriologist, about the ALS culture test:

"This is a scam if there ever was one"

Quote from Gigi Jordon(Mirra's ex-wife and business partner):

Jordon says she can prove that Mirra is also involved in an even grander prescription drug scam."That $274 million involving Allion is just the tip of the iceburg"Jordon claims."The black market hemophillia operation Rays involved in brings in billions a year.He knew that I knew what he was doing and so he needed to neutralize me".

Re: Improved Borrelia burgdorferi Blood culture Method

Posted: Tue 19 Feb 2013 21:21
by TicksSuck
The Shills have become hyperactive http://www.lymeneteurope.org/forum/view ... net+shills.

Bagge, you make me [sic]k! :mrgreen:

Let's not get distracted from the subject of a legitimate research paper being reported, with important results which should stand on their own. Maybe, instead of focusing on conspiracies and association with member of the Mafia, some of you could read the paper and note that nowhere is there mention that the subjects in the study ever have been treated for Lyme disease in the first place. Wouldn't this be a more important observation to post here if you were really interested in Lyme disease research (and were not just shill'in)?

It might be that the subjects had "properly" been treated before, but it is not mentioned anywhere. Why is that? If it was the case, the implication of it would have made this worth a mention, wouldn't you think?

No need to reply to me with links to Mirra and all. You made you point in other threads and repeating it here doesn't make it any more relevant!