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band KDa 20 and 29

Posted: Sat 17 Jan 2015 21:22
by hansemand
Are these bands specific for any Borrelia spp?

Re: band KDa 20 and 29

Posted: Sun 18 Jan 2015 6:22
by LHCTom
On what Labs Western Blot were they indicated or reported? Do you have the Blot?

20kDa is between DbpA (17-19) and OspC (21-23).

29kDa is between OspD (28) and 30 kDa which indicated in "Borrellia Molecuar Biology, Host Interactions and Pathogenensis" as distinct and not named and may just be a OspA ( 31).

You need to understand that Borrelia has many dozens of surface antigens/proteins and each has many possible epitopes and therefore legitimate antibodies.

The 10 chosen and their associated antibodies are just a small subset. Different strains and species can have different weights as in the case of OspC which can range from 21-25 kDa.

Then there are the actual source of the antigens chosen in the Western Blot test design and the calibration of the weight scale.

So they are very close to the chosen antigens so 20 kDa might be DbpA (17-19) or OspC (21-23) while 29 might be 30 or OspA.

Re: band KDa 20 and 29

Posted: Sun 18 Jan 2015 7:06
by hansemand
Thank you for the answer.
Old westernblots from laboratoriums Medidizin in Koln
3 blot were made with positive Elisa, band 41 in all, and 29, 39 and 20 in the seperate tests.
At that time the lab obviously Idsa rules and descibed the 20 and 29 negative, and I was uninformed.

Re: band KDa 20 and 29

Posted: Sun 1 Feb 2015 17:51
by hansemand

Re: band KDa 20 and 29

Posted: Sun 1 Feb 2015 19:22
by ChronicLyme19
Results: 27 different non-specific bands were detected in both groups. Six of 27 (22%) of the non-specific bands were detected significantly more in the western blot positive patients compared to the western blot negative patients (20 kDa, p<0.0001; 28 kDa, p<0.002; 36 kDa, p<0.002; 37 kDa, p<0.007; 48 kDa, p<0.023; 56 kDa, p<0.028; two-tailed F test).
Correlation doesn't necessarily mean causation though. How do we know these are actually Lyme antibodies, rather than antibodies to something that commonly co-infects with Lyme?