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Borrelia Spirochetes by FISH in Alzheimer's- an Atlas

Posted: Mon 9 Mar 2015 14:43
by inmacdonald
An Atlas of Images of Borrelia burgdorferi (sl) group of Spirochetes
in High Resolution DNa In Situ Hybridization studies
of Hippocampus sections from Alzheimer's Disease Braak VI

All are formalin fixed, Paraffin Embedded sections,
deparaffinized, and passed through serial histology laboratory solutions
in descending order : Xylene, Xylene, Xylene, 100% alc,100% alc,100% alc,
90% alc, 80% alc, 70% alc ,50% alc, H20.

Air dried sections are layered with Borrelia burgdorferi specific
Molecular Beacon DNa Probe-FITC.
Hybridization at 60 deg c in hybridization buffer ( No DMF needed for Molecular beacons)
slides then washed in distilled H20 to remove any unhybridized DNA probes.
DNA Probe : Molecular beacon -FITC Fluor/DABCYL quencher
TGG GAG TTT CTG GTA AGA TTAA
EpiFluorescence microscopy at 40x and 100x magnification
Image Capture with a high resolution 18 megapixel camera.

Link to Images: An Atlas of Borrelia spirochetes imaged from Harvard Brain Bank Alzheimer's disease accessions(5/5)
https://www.dropbox.com/s/rlxv1ibmi5jrw ... k.pdf?dl=0

CONCLUSION:
Fluorescent In Situ DNA Hybridization ( FISH method) using Borrelia specific DNA probes ( BBO 0147-Flagellin B)
results in direct Imaging of Borrelia Spirochetes in the Human Hippocampus
5 new cases from the Harvard Brain Bank are positive (5/5 + by FISH )
Alzheimer's disease Braak VI established by Neuropathologists at Harvard in 5/5 cases.


Respectfully,
Alan B. MacDonald MD, FCAP
March 9,2015

Re: Borrelia Spirochetes by FISH in Alzheimer's- an Atlas

Posted: Wed 18 Mar 2015 4:00
by LHCTom
Alan,

The microphotographs are quite impressive. Are these are from Alzheimers brains from the Harvard collection? Are there other Harvard researchers involved? Alzheimers is possibly the most significant disease as Baby Boomers age besides cancer. I would think these discoveries would really get the attention of Alzheimers research and could be explosive. Maybe you could say something about the reaction and interest this is generating?

Re: Borrelia Spirochetes by FISH in Alzheimer's- an Atlas

Posted: Wed 18 Mar 2015 20:48
by ChronicLyme19
I second LHCTom's comment. This is really neat stuff. It brings up a ton of questions tho...

How do "normal" brains look in comparison? Are they 100% free of spirochetes? Do they have some, but not as many as these brains? Are borrelia spirochetes the only bug that can be found in these brains?