Antibacterial Amyloid-Coats circulating Borrelia Biofilms in Blood

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inmacdonald
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Joined: Fri 13 Jan 2012 22:32

Antibacterial Amyloid-Coats circulating Borrelia Biofilms in Blood

Post by inmacdonald » Thu 8 Oct 2015 15:41

Amyloid - as an Antibacterial agent - Paired with DNA Probe study to localize Bioflms of borrelia in Alzheimer's Brain tissue:

Alzheimer's Disease Brain Study - 1000 amyloid Plaques examined.
Use DNA probes for Borrelia miyamotoi to examine Autopsy Brain sections from 6 cases of Alzheimer's Disease
from the Harvard University Brain Tissue Resource Center ,Belmont, Mass [ McClean Hospital Brain Bank}
and then perform amyloid stains on the identical slides to localize the exact site of the Alzheimer's plaques.

Link: Link: https://vimeo.com140175819 a narrated video powerpoint lecture - one hour of detailed methods and results



Circulating biofilms of miyamotoi Borrelia in a 62 year old woman with Dementia:

Link:
https://www.dropbox.com/s/s5qqoxv5stq04 ... 9.pdf?dl=0


Circulating Amyloid Coated Biofilms in this 62 year old woman's peripheral blood:
Link:
https://www.dropbox.com/s/wza01fpuqovy9 ... d.pdf?dl=0



Conclusion: in Alzheimer's Disease, Dr. Rudolph Tanzi PhD has established that beta 1-42
Amyloid has significant Antibacterial Activity. [ Method: Extract Beat 1-42 amyloid
from the temporal lobes of Autopsy Alzheimer's disease Brains. Add the brain
extract to laboratory cultures of microbes. Observation: Microbial growth
is arrested by the Brain Amyloid extracts.]
Link:
http://journals.plos.org/plosone/articl ... ne.0009505

Corroboration October 2015: A paired study of 100 Amyloid plaques from 6 cases of Alzheimer's and one
living patient with Dementia -volunteer to provide peripheral blood
for DNA FISH hybridization and follow-up Amyloid stains of blood smears.
Disease : 1. FISH method DNA probes allowed to seek out Borrelia miyamotoi DNA
2. DNA probe Hybridization performed - ( probes carry Cy5 label--[ a fluorochromes emitting red]
3. Stain the same slide After FISH hybridization with Borrelia miyamotoi DNA probes
with amyloid Stain ( Congo Red stain works well, Thioflavin T (green) stain works well]
4. Photograph one amyloid plaque under White polarized light { Photo "A"}
5. Lock the microscope ; locked field of view/ Locked plane of focus
6. Turn off the white light
7.Turn on the monochrome light to produce Cy5 fluorescence
8. Take a second photograph {Photo "B"}
9 Compare Photo "A" with Photo "B"
10 . Repeat steps 1-9 for 1000 Alzheimer Amyloid plaques
11. Tabulate the Results.
12. Obtain peripheral blood from 62 year old patient with Dementia, use FISH method
and Miyamotoi DNA probes to examine blood for circulating Biofilms of borrelia
( miyamotoi and burgdorferi), then stain the peripheral blood with Congo red stain
for Amyloid. Photodocument all positive fields of view.
13.. Offer the manuscript for publication

Respectfully,
Alan B. MacDonald, MD ,FCAP
October 8,2015

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inmacdonald
Posts: 977
Joined: Fri 13 Jan 2012 22:32

Re: Antibacterial Amyloid-Coats circulating Borrelia Biofilms in Blood

Post by inmacdonald » Wed 18 Nov 2015 17:54

Images of Particulate Amyloid Clumps in Human peripheral blood Smear -coating Circulating Borrelia Biofilms
Biofilm Borrelia A & B  with Blood Biofilm amyloid.jpg
Comparison of Amyloid Brain Plaques, Biofilm of Borrelia inside the Amyloid brain Plaques, and Amyloid Clump (Rigid Particle of Amyloid Coated Borrelia biofilm ) in the circulating blood.
Biofilm Borrelia A & B with Blood Biofilm amyloid.jpg (149.31 KiB) Viewed 1483 times
Dementia blood Amyoid 3 water channels jpg.jpg
Amyloid polymer is stained red with Congo Red Stain. Birefringence with polarized light is demonstrated in the Congo Red Stained solid material ,flanking the empty spaces in the Amyloid plaque.
Dementia blood Amyoid 3 water channels jpg.jpg (163.58 KiB) Viewed 1483 times

-----
Comment: this is the First demonstration of Particulate (insoluble) amyloid polymer coating Borrelia biofilm communities
which are circulating in the peripheral blood. The patient is Living with Dementia, and is markedly impaired by the dementia.
She has lost much of her ability to speak and to comprehend speech. The Actual Anatomic Cause of the Dementia in this patient
cannot be determined while she is alive. Only A thorough Brain autopsy with specialized Neuropathology studies can definitively
name the type of Dementia in this patient.


Polymers of Amyloid ( a naturally self-polymerizing protein) form sheets with a Beta pleated structure.
Amyloid Stains routinely used in tissue deposits of Amyloid include : 1. Congo Red Stain, 2. Thioflavin T Stain, 3 and various
immunostains for Specific types of Amyloid.

The Amyloid in Alzheimer's Disease is Beta 1-42 preponderant amyloid, but Alzheimer's disease also demonstrates other
structural variations of Beta Amyloids.
In Nature, Amyloid is produced by: Bacteria, plants, and Animals.
In the human, over 100 different Amyloid types have been described.
Endocrine Amyloid is exemplified by the Medullary Carcinoma of the Thyroid amyloid deposits.
Diabetic Amyloid is derived from insulin precursors.
Multiple myeloma amyloid is derived from polymers of immunoglobulin light chains.

Because Amyloid polymers are : 1.Insoluable, 2. Resistant to any enzymatic Degradation in vivo, and 3. Are Rigid-
the consequence of circulating Amyloid Clumps in the blood of some dementia patients sets the stage
for Embolic behavior by the microscopic Amyloid particles. Emboli have the ability to injure tissues
by depriving the Tissue cells of oxygen transferred to the cells across capillaries from red blood cell release
of oxygen to the peripheral tissues.
If Capillary vessels or if arterial supply of blood to a tissue site is blocked by a rigid Embolus.
the tissue will be injured, and my undergo micro infarction ( cell death)

Respectfully,
Alan B. MacDonald, M.D. FCAP
November 18,2015
Attachments
Dementia blood Biofilm Miyamotoi.jpg
Biofilm communities in Blood smear FISH method DNA probes for Miyamotoi borrelia,
in a living patient with Borrelia Dementia.
Dementia blood Biofilm Miyamotoi.jpg (152.57 KiB) Viewed 1483 times

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