Borrelia Burgdorferi in Testiclular tissue by ImmunoStains with controls

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inmacdonald
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Borrelia Burgdorferi in Testiclular tissue by ImmunoStains with controls

Post by inmacdonald » Tue 17 Nov 2015 15:08

A Microscopic Study of Male Gonad for detection of Borrelia burgdorferi by Alan B. MacDonald, MD


Immunoperoxidase staining for Borrelia burgdorferi:
Tissues: Autopsy Testicle from a complete Autopsy Case from an adult male who died in Minnesota after
Chronic Lyme Borreliosis. All Organs including Autopsy Brain were positive for Borrelia Burgdorferi
Controls: Animal Tissue containing Borrelia burgdorferi spirochetes { Mouse} - POSITIVE Control
Negative controls: Laboratory Mouse tissue - not Containing Borrelia burgdorferi
By immunostains specific for detection of Borrelia Burgdorferi
Annotated - Human Testicle with Borrelia Burgdorferi in tissue and Intracellular sites.jpg
Human Adult Autospy Testicle with Abundant Borrelia burgdorferiby Immunoperoxidase stain
Annotated - Human Testicle with Borrelia Burgdorferi in tissue and Intracellular sites.jpg (379.39 KiB) Viewed 3030 times

Control slide - Borrelia burgdorferi - Immunohistochemistry with Antibody to B. burgdorferi - and AEC chromogen (RED).jpg
Tissue Control ( Mouse Testilce) Positve for Borrelia burgdorferi by immunoperoxidase stain
Control slide - Borrelia burgdorferi - Immunohistochemistry with Antibody to B. burgdorferi - and AEC chromogen (RED).jpg (275.7 KiB) Viewed 3030 times

------------------------------------------------
Formulation:

Testicle of the Rabbit was used to keep Treponema pallidum alive in19th century laboratories.
Spirochetal Control Stains commercially available , since the early 20th century, have been , Testicle tissue from Syphilitic Rabbits. No Other spirochetal control tissues have been available for purchase for hospital histology laboratories to use as positive control materials for
silver impregnation methods to stain spirochetes in tissues.
Recently, the demand for tissue controls for use in Immunohistochemistry staining for borrelia spirochetes in tissue, has
been satisfied by the use of Mouse tissue- Specifically Mouse TESTICLE tissue ONLY
.
All other Autopsy mouse tissues are
inferior to Mouse testicle for POSITIVE tissue Controls for staining Borrelia in human tissue studies.

Assessment: Borrelia persist in Chronically infected mammalian hosts.
The anatomic Sites of Borrelia Persistence -- Are " SANCTUARY SITES"
Sanctuary Sites in the mammalian host for Chronic "Deposits" of Borrelia - in persister form are:
BRAIN
EYE
TESTICLE


Seminal fluid consists of Sperm, Spermatic Fluid, and from the Seminal Vesicles; Seminiferous Fluid.

By Any Yardstick, A borrelia Infected Testicle, in a Chronically Diseased patient , has an enormous number
of Infectious Borrelia { high density borrelia infection]

Ejaculates from Chronically Borrelia Infected men are Filled with Virulent, Living borrelia,
and these are fully capable of transmission of borrelia Infection to
the Conjugal partner.

In retrospect, the Legal Requirement for Premarital "Blood Testing" commencing in the 19th and continuing through
the early 20th Century, was predicated on the Public Health Laws to prevent Syphilis infection from
passing between marital partners,[ FIRST} and to prevent Transmission of Syphilis infection from the
parents to the Unborn Fetus in the Uterus [Second ]

An person who disputes the Reality of the Transmission of Virulent Spirochetal Infections
between conjugal partners is an Un-informed FOOL., who missed the Medical School lectures
on syphilis and "BAD BLOOD"

Respectfully,
Alan B. MacDonald, MD, FCAP
November 17, 2015
Attachments
Annotated - Human Testicle with Borrelia Burgdorferi in tissue and Intracellular sites.jpg
Human Autospy Testicle with borrelia burgdorferi by immunoperoxidase stain
Annotated - Human Testicle with Borrelia Burgdorferi in tissue and Intracellular sites.jpg (379.39 KiB) Viewed 3030 times

hv808ct
Posts: 256
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Re: Borrelia Burgdorferi in Testiclular tissue by ImmunoStains with controls

Post by hv808ct » Tue 17 Nov 2015 20:44

Borrelia Burgdorferi in Testiclular tissue by ImmunoStains with controls
Post by inmacdonald » Tue 17 Nov 2015 15:08

By Any Yardstick, A borrelia Infected Testicle, in a Chronically Diseased patient , has an enormous number of Infectious Borrelia { high density borrelia infection]

Ejaculates from Chronically Borrelia Infected men are Filled with Virulent, Living borrelia,
and these are fully capable of transmission of borrelia Infection to the Conjugal partner.

An person who disputes the Reality of the Transmission of Virulent Spirochetal Infections
between conjugal partners is an Un-informed FOOL., who missed the Medical School lectures on syphilis and "BAD BLOOD"
Imagine all the thousands of “Un-informed FOOL” infectious disease experts and epidemiologists who don’t realize this startling ‘fact’. It’s a good thing there are public web sites where these kinds of medical/infectious disease breakthroughs can be announced to the world. Otherwise we all might have to rely on peer-reviewed publications and confirmatory experiments.

Probably this puts me in the “Un-informed FOOL” class, but I heard those lectures and gave similar lectures to undergrads, and I still don’t understand the relationship between syphilis and borreliosis…unless morphology is everything. Maybe I’m just paying too much attention to a century’s worth of epidemiology. Or maybe those color-code images just look like a lot of debris, stain precipitates, and Lowell-like wishful thinking. Could we get some expert 2nd opinions about those images? Would that be too radical?

duncan
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Re: Borrelia Burgdorferi in Testiclular tissue by ImmunoStains with controls

Post by duncan » Tue 17 Nov 2015 21:56

Public web sites are responsible for the dismantling of the PACE Trial results, and potentially could lead to the end of the BPS school of thought. No more psych crutches for inadequate or biased medical research. That includes many mainstream Lyme pushers of "blame-the-patient" ideology.

You should read up on it while on the Internet, hv808ct. It is a fascinating read.

Not all public websites are good. But enough are to make changes where change is desperately needed.

Public web sites can emerge as the new paradigm that help expose fraud and misinformation, and can mark the decline of misguided and harmful dogma wherever it surfaces or lurks entrenched, as its flaws and prejudices get a healthy dose of reasoned logic.

As Shakespeare opined: The truth will out.

Public web sites can just out it all the faster, and in concrete fashion.

admin
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Re: Borrelia Burgdorferi in Testiclular tissue by ImmunoStains with controls

Post by admin » Tue 17 Nov 2015 22:42

In which medical journal was this published?

nnecker
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Joined: Wed 19 Dec 2012 22:57

Re: Borrelia Burgdorferi in Testiclular tissue by ImmunoStains with controls

Post by nnecker » Wed 18 Nov 2015 10:49

Here is another autopsy done by Dr Mack.He found Borrelia in this one as well.

https://www.youtube.com/watch?v=P1FJQT0V7gY

hv808ct
Posts: 256
Joined: Wed 30 Jul 2008 4:11

Re: Borrelia Burgdorferi in Testiclular tissue by ImmunoStains with controls

Post by hv808ct » Wed 18 Nov 2015 20:19

Re: Borrelia Burgdorferi in Testiclular tissue by ImmunoStains with controls
Post by duncan » Tue 17 Nov 2015 21:56

Public web sites are responsible for the dismantling of the PACE Trial results, and potentially could lead to the end of the BPS school of thought. No more psych crutches for inadequate or biased medical research. That includes many mainstream Lyme pushers of "blame-the-patient" ideology
I thought this post was about the dubious assertions of a connection between testicles, borrelia and STIs. I’m not sure what CFS or PACE or BPS (no idea what this is) has to do with anything though I did read the Lancet response to the “public web site” outrage over PACE. (The Lancet comment is posted below. At least read the first and last sentences.) I think the original point here was that if you’re going to make some fantastic medical or scientific claim, you don’t do it on the digital equivalent of public access television. You do in the pages of a journal where your peers can read it and then take the M&M section and repeat it.


Once every few years, we publish a paper that elicits an outpouring of consternation and condemnation from individuals or groups outside our usual reach. The latest topic to have caused such a reaction is chronic fatigue syndrome (CFS), and—more specifically—Peter White and colleagues’ randomised PACE trial published on March 5, this year.

In the PACE trial, White and colleagues set out to answer a question that has long troubled the CFS community: are the treatments recommended by clinical guidelines—ie, cognitive behaviour therapy and graded exercise therapy—really the best option for patients with CFS? The trial’s findings showed that, compared with specialist medical care alone, both treatments were associated with significant improvements in self-rated fatigue and physical function (the primary outcomes) after 52 weeks. The response to the trial’s publication was swift and damning. “When is the Lancet going to retract this fraudulent study?” demanded a Facebook group. A 43-page complaint (now available via Wikipedia) branded the trial “unethical and unscientific”.

There were 44 formal letter submissions, eight of which we publish today, together with a response from White and colleagues. Many of the letters critique the definitions of secondary outcomes, question protocol changes, and express concern over generalisability. But one cannot help but wonder whether the sheer anger and coordination of the response to this trial has been born not only from the frustration many feel about a disabling condition, but also from an active campaign to discredit the research.

White and colleagues have been accused of having “formed their opinion about the intended outcome” before the trial began. This view is unjustified and unfair. The researchers should be praised for their willingness to test competing ideas and interventions in a randomised trial. The evidence might even suggest that it is the critics of the PACE trial who have formed their opinions first, ignoring the findings of this rigorously conducted work. ■ The Lancet

duncan
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Joined: Wed 5 Sep 2012 18:48

Re: Borrelia Burgdorferi in Testiclular tissue by ImmunoStains with controls

Post by duncan » Wed 18 Nov 2015 20:36

Sloppy, hv808ct.

Look up David Tuller and James Coyne and their open examinations (within the last month) of the PACE Trial results. In particular, review Tuller's (of the NY Times) exhaustive critique.

Look up the recent petition - signed by over 10,000 patients (PACE authors had characterized patient criticisms as limited to a small vocal minority) in less than two weeks - requesting Lancet retract the PACE paper.

Look up the letter signed by leading US scientists requesting an independent investigation into PACE.

Read the - to date - what many feel are wholly inadequate responses offered up by the PACE authors; take a close look at their inability to rebut the comprehensive and compelling and precise dismantling of not just PACE findings, but of the methodology they used.

It's a train wreck unfolding.

You should know that. That you don't...

hv808ct
Posts: 256
Joined: Wed 30 Jul 2008 4:11

Re: Borrelia Burgdorferi in Testiclular tissue by ImmunoStains with controls

Post by hv808ct » Thu 19 Nov 2015 0:00

Re: Borrelia Burgdorferi in Testiclular tissue by ImmunoStains with controls
Post by duncan » Wed 18 Nov 2015 20:36

Sloppy, hv808ct.

Look up David Tuller and James Coyne and their open examinations (within the last month) of the PACE Trial results. In particular, review Tuller's (of the NY Times) exhaustive critique.
Well, as noted, these posts are supposed to be about testicles and borrelia and fantastical STIs when the first two meet. I know little about CFS and even less about any recent UK-funded trials. On the other hand, The Lancet has been around for 2 centuries. I think they know what they’re doing.

duncan
Posts: 1370
Joined: Wed 5 Sep 2012 18:48

Re: Borrelia Burgdorferi in Testiclular tissue by ImmunoStains with controls

Post by duncan » Thu 19 Nov 2015 0:47

Well, if you think they know what they're doing... :)

Do you think the IDSA Lyme peeps know what they are doing, too?

Have they participated in any meaningful studies in the last five years on late stage disseminated Lyme manifesting in testes, for instance? Not counting retrospectives? Has the NIH?

If you feel compelled to take pot shots at this thread's topic, can you point to recent meaningful research that wasn't just a counterpoint to similar unilateral efforts?

hv808ct
Posts: 256
Joined: Wed 30 Jul 2008 4:11

Re: Borrelia Burgdorferi in Testiclular tissue by ImmunoStains with controls

Post by hv808ct » Thu 19 Nov 2015 15:23

Re: Borrelia Burgdorferi in Testiclular tissue by ImmunoStains with controls
Post by duncan » Thu 19 Nov 2015 0:47

Have they participated in any meaningful studies in the last five years on late stage disseminated Lyme manifesting in testes, for instance? Not counting retrospectives? Has the NIH?
If you feel compelled to take pot shots at this thread's topic, can you point to recent meaningful research that wasn't just a counterpoint to similar unilateral efforts?
There isn’t any reason to waste money on such activities. It would be like checking periodically that the earth is a sphere. It is.

Lyme Borreliosis is not Sexually Transmitted.
http://aldf.com/wp-content/themes/ALDF/ ... .10.14.pdf

Is Lyme disease sexually transmitted?
http://www.aldf.com/lyme-disease/#misinformation

And while we’re on the subject of fantastic etiologies….

Does Lyme disease play a significant role in the etiology of Alzheimer’s disease?
http://www.aldf.com/lyme-disease/#misinformation

Now before someone points out that I’m just referencing another opinionated web site, I’m not. I’m referencing short summaries with excellent references back to the primary literature, which can be found through PubMed.

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