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Glioblastoma multiformae 5 cases positive for Burgdorferi borrelia DNA

Posted: Thu 21 Jan 2016 14:26
by inmacdonald

Glioblastoma Multiformae [GBM] contains DNA of Borrelia burgdorferi/in tumor cells

First report in the world Literature by Alan B. MacDonald,M.D.

Fluorescence In situ DNA Hybridization of Brain biopsies with DNA probe
to gene bbo 0740, which codes for Borrelia family [ Burgdorferi group (sl) INNER CELL MEMBRANE

Link:
PDF image file of results from Five of Five GBM cases

Please navigate to the website below:
http://www.durayresearch.wordpress.com

Respectfully,

Alan B. MacDonald, M.D., FCAP
Jan 21,2016

Re: Glioblastoma multiformae 5 cases positive for Burgdorferi borrelia DNA

Posted: Fri 22 Jan 2016 22:07
by inmacdonald
Glioblastoma Multiformae {GBM} Astrocytoma ( Malignant) Grade 4/4; Oncogenic Effect of Borrelia DNA on Human Glial Cells

Bacterial and viral Oncogenesis - past and future models - Notes from a Borrelia Watcher


Infection driven neoplasias have been open territory

for new discoveries in the events which produce

cancers in mankind.


Most notably since the discovery of links between


Helicobacter pylori and Gastric Lymphomas and


adenocarcinomas, and HPV induced neoplasias from

mouth to anal domains, chronic infections have provided

a model to explain the transit of a benign cell into a

malignant cell.





Neoplasias may be driven by infection related


chronic infections, but we in pathology have hard data


sets to show that only Specific DNa of Infectomes are capable of


neoplastic alteration of human DNA to produce


neoplasias.





In the Case of Neurons, Glial cells and Borrelia,


there is evidence from co-incubation of cells


in tissue culture with living Borrelia in pure


cultures, that Borrelia first binds to the tissue


cultured cells, and then, stepwise , inserts the entire


spirochete into the Cytoplasm and then into the nucleus


of Glial cells. Liposomes from Borrelia's outer surface


membrane contain DNa. Liposomal Transfection is


a commercially available tool for those who wish to


transfect cells in tissue culture. Transmission


electron micrographs have imaged the transit of


borrelia liposomes from the external cellular


milieu, to penetrate cytoplasm of eukaryotic cells,


and next to penetrate the nucleus.


I have published in year 2006 Transfection DNa


with Borrelia flagellin DNa inserted into human


chromosome 11q in seven cases of Alzheimer's


disease autopsy tissue from total DNA extracts

of Fresh AD hippocampus. The year 2006 Transfection

nucleotide (DNA) sequences were carefully reviewed

by experts at GenBank, the National Repository for

DNA sequences for the USA and Worldwide.

Not every submitted new DNa sequence submitted to

GenBank is accepted for "Deposit" into the GenBank.

fortunately, all seven of my transfection DAN sequences

were found to be proper for GenBank deposit,

and these are now Immortalized on the Internet

in the NCBI Website/ GenBank .








And so it has been on my list of dream projects


for many years, to attempt to find Borrelia Dna


in Central Nervous System Neoplasias.




Today, I discovered that tissues from the most lethal of


primary brain tumors-GBM- [ Glioblastoma Multiformae]


contain DNA of borrelia burgdorferi (sl) family of microbes


in five of five cases. This project ahs been 25 years in the


"hatching" from nascent inspiration based on the


knowledge that borrelia can penetrate any cell in the human


body at will, and thereafter, set up residence inside the cell's


compartments [ cytoplasmic region or the nuclear compartment]


I demonstrated in year 2006 that borrelia transfection of


human DNA occurred in seven of ten Alzheimer's disease whole


brain DNA extractions.




Livengood and Gilmore, scientists on the payroll

of the Centers for Disease Control and Prevention,

published their In vitro study of Borrelia penetration

of Human Neurons and human benign Glial cells.

They used an in vitro [ tissue culture} model to co-incubate


living neuron cells in tissue culture medium with live borrelia. The borrelia


adhered to the Neurons, and subsequently invaded the cell's


inner compartments. Glia cells in tissue culture, co-incubated

with live borrelia demonstrated the identical Cell invasive behavior.




Link:

https://ogcloud.gonitro.com/p/xm9Y.hauAjANgEt265wPN





Notes on previous borrelia transfection behaviors:





Transfection is the phenomenon of foreign DNa inserting itself


into the DNa of another life form. When the cells are eukaryotic


from man or from mammals, when the borrelia penetrates the


nucleus of the cell, it has free access to wreak havoc with


mammalian DNA. Transfection inserts produce mutations


which encompass "readable" nucleotide sequences still recognizable


as borrelial DNA sequences inside of the DNA sequences of native


human DNa. I developed the notion that transfected mammalian benign glial cells


of the brain and spinal cord, could be rendered immortal and


cancerous. Therefore, the concept of infection initiated brain glial


tumors was hatched.





The FISH method, is in situ DNA hybridization of a DNA probe


to a target family of heterogeneous DNA molecules.


FISH method study demonstrated a huge number of borrelia DNA

fragments in Glioblastoma Multiformae tumor cells.




This, to my personal analysis , speaks for Borrelia penetration

of benign glial cells, initially penetration of the Glial cell cytoplasm,

and subsequently, penetration of the Nucleus of the Glial cells.

Borrelia DNA is then present in the Only naturally occurring

DNA containing compartment of the Benign Glial cells of the human

brain. Over time, I believe, that the borrelia DNA inserts into the

human chromosomes of the glial cell. Post Insertion of borrelia DNA

into Glial cells, malignant Transformation of glial cells occurs,

and an Astrocytoma is "borne". The degree of aggressiveness

of Astrocytomas is numerically grouped into four Grades.

Grade 4 Astrocytomas are the most aggressive and therefore

the most malignant primary brain tumor known to man. Glioblastoma

Multiformae [ GBM] is a grade 4 Astrocytic malignancy.







Bacterial DNA has no legitimate place inside of Glioblastoma

Multiformae tumor cells. The molecular mechanism behind the transformation of

infected but benign human eukaryotic cells, and malignant

human eukaryotic cells harboring infection of any kind

[ be it Viral oncogenesis or be it bacterial oncogenesis]

is yet to be worked out in detail. Helicobacter pylori

infections in some human hosts are responsible for

the development of Lymphoid malignancies ( Malignant Lymphomas]

and in some infected patients, the development of

Epithelial cell malignancies ( Adenocarcinomas)




Mutations are a common event in transformation of

benign cells into fully malignant cells.

Bacterial infection now has a legitimate place

in some categories of human oncogenesis, in

exactly the same thread that certain viral infections

of human cells may produce cancers in some patients.




Our mission in garnering greater support for

bacterial infection initiated oncogenesis in the

human host, is to eliminate the notion that

only viral infections can serve as oncogenic

events in the human host ( i.e. Human Papilloma virus

[ HPV ] types 16, and 18, Hepatitis C viral subtypes,

Epstein Barr viral infections and Burkitt lymphomas.

Rous Sarcoma virus and malignancies, .. and the list goes

on)

LINK:
https://ogcloud.gonitro.com/p/_skzEnGqeLIw_qY4yy1X8W




Respectfully,

Alan B. MacDonald, M.D.

Fellow, College of American Pathologists

January 23, 2016

Re: Glioblastoma multiformae 5 cases positive for Burgdorferi borrelia DNA

Posted: Fri 22 Jan 2016 22:29
by inmacdonald
Statistical FACTS; Glioblastoma Multiformae( Astrocytoma Grade 4) from Wikipedia

Incidence: 5 to 7 persons per 100,000 population
Age of Peak Incidence: 55 years old
Survival: All therapies averaged - 3 months from time of Diagnosis by tissue Biopsy ( Brain Biopsy)
Reference: Wikipedia.Com\

Note; Incidence of primary Brain Malignancies : Approximately identical - Across All Decades of Life { all tumor types Averagedin this statistic]
Note: Brain Tumors are ALWAYS biologically Malignant, Even if the microscopic findings are not those of a virulent Cancer; Viz; Meningioma : a Primary Brain tumors which are ALWAYS Biologically MALIGNANT; in spite of the Fact that Meningiomas
never metastasize beyond the confines of the skull..

Astrocytomas Grade 4 {Glioblastoma Multiformae- GBM] - Highly malignant - mean survival after diagnosis = 3 months
Exceptional cases of GB MAY M survive longer than 3 months after Tissue diagnosis { Sen. Edward M. Kennedy]



Link:
https://ogcloud.gonitro.com/p/xm9Y.hauAjANgEt265wPN



Infection driven neoplasias have been open territory

for new discoveries in the events which produce

cancers in mankind.


Most notably since the discovery of links between


Helicobacter pylori and Gastric Lymphomas and


adenocarcinomas, and HPV induced neoplasias from

mouth to anal domains, chronic infections have provided

a model to explain the transit of a benign cell into a

malignant cell.





Neoplasias may be driven by infection related


chronic infections, but we in pathology have hard data


sets to show that only Specific DNa of Infectomes are capable of


neoplastic alteration of human DNA to produce


neoplasias.





In the Case of Neurons, Glial cells and Borrelia,


there is evidence from co-incubation of cells


in tissue culture with living Borrelia in pure


cultures, that Borrelia first binds to the tissue


cultured cells, and then, stepwise , inserts the entire


spirochete into the Cytoplasm and then into the nucleus


of Glial cells. Liposomes from Borrelia's outer surface


membrane contain DNa. Liposomal Transfection is


a commercially available tool for those who wish to


transfect cells in tissue culture. Transmission


electron micrographs have imaged the transit of


borrelia liposomes from the external cellular


milieu, to penetrate cytoplasm of eukaryotic cells,


and next to penetrate the nucleus.


I have published in year 2006 Transfection DNa


with Borrelia flagellin DNa inserted into human


chromosome 11q in seven cases of Alzheimer's


disease autopsy tissue from total DNA extracts

of Fresh AD hippocampus. The year 2006 Transfection

nucleotide (DNA) sequences were carefully reviewed

by experts at GenBank, the National Repository for

DNA sequences for the USA and Worldwide.

Not every submitted new DNa sequence submitted to

GenBank is accepted for "Deposit" into the GenBank.

fortunately, all seven of my transfection DAN sequences

were found to be proper for GenBank deposit,

and these are now Immortalized on the Internet

in the NCBI Website/ GenBank .








And so it has been on my list of dream projects


for many years, to attempt to find Borrelia Dna


in Central Nervous System Neoplasias.




Today, I discovered that tissues from the most lethal of


primary brain tumors-GBM- [ Glioblastoma Multiformae]


contain DNA of borrelia burgdorferi (sl) family of microbes


in five of five cases. This project ahs been 25 years in the


"hatching" from nascent inspiration based on the


knowledge that borrelia can penetrate any cell in the human


body at will, and thereafter, set up residence inside the cell's


compartments [ cytoplasmic region or the nuclear compartment]


I demonstrated in year 2006 that borrelia transfection of


human DNA occurred in seven of ten Alzheimer's disease whole


brain DNA extractions.




Livengood and Gilmore, scientists on the payroll

of the Centers for Disease Control and Prevention,

published their In vitro study of Borrelia penetration

of Human Neurons and human benign Glial cells.

They used an in vitro [ tissue culture} model to co-incubate


living neuron cells in tissue culture medium with live borrelia. The borrelia


adhered to the Neurons, and subsequently invaded the cell's


inner compartments. Glia cells in tissue culture, co-incubated

with live borrelia demonstrated the identical Cell invasive behavior.




Link:

https://ogcloud.gonitro.com/p/xm9Y.hauAjANgEt265wPN





Notes on previous borrelia transfection behaviors:





Transfection is the phenomenon of foreign DNa inserting itself


into the DNa of another life form. When the cells are eukaryotic


from man or from mammals, when the borrelia penetrates the


nucleus of the cell, it has free access to wreak havoc with


mammalian DNA. Transfection inserts produce mutations


which encompass "readable" nucleotide sequences still recognizable


as borrelial DNA sequences inside of the DNA sequences of native


human DNa. I developed the notion that transfected mammalian benign glial cells


of the brain and spinal cord, could be rendered immortal and


cancerous. Therefore, the concept of infection initiated brain glial


tumors was hatched.





The FISH method, is in situ DNA hybridization of a DNA probe


to a target family of heterogeneous DNA molecules.


FISH method study demonstrated a huge number of borrelia DNA

fragments in Glioblastoma Multiformae tumor cells.




This, to my personal analysis , speaks for Borrelia penetration

of benign glial cells, initially penetration of the Glial cell cytoplasm,

and subsequently, penetration of the Nucleus of the Glial cells.

Borrelia DNA is then present in the Only naturally occurring

DNA containing compartment of the Benign Glial cells of the human

brain. Over time, I believe, that the borrelia DNA inserts into the

human chromosomes of the glial cell. Post Insertion of borrelia DNA

into Glial cells, malignant Transformation of glial cells occurs,

and an Astrocytoma is "borne". The degree of aggressiveness

of Astrocytomas is numerically grouped into four Grades.

Grade 4 Astrocytomas are the most aggressive and therefore

the most malignant primary brain tumor known to man. Glioblastoma

Multiformae [ GBM] is a grade 4 Astrocytic malignancy.







Bacterial DNA has no legitimate place inside of Glioblastoma

Multiformae tumor cells. The molecular mechanism behind the transformation of

infected but benign human eukaryotic cells, and malignant

human eukaryotic cells harboring infection of any kind

[ be it Viral oncogenesis or be it bacterial oncogenesis]

is yet to be worked out in detail. Helicobacter pylori

infections in some human hosts are responsible for

the development of Lymphoid malignancies ( Malignant Lymphomas]

and in some infected patients, the development of

Epithelial cell malignancies ( Adenocarcinomas)




Mutations are a common event in transformation of

benign cells into fully malignant cells.

Bacterial infection now has a legitimate place

in some categories of human oncogenesis, in

exactly the same thread that certain viral infections

of human cells may produce cancers in some patients.




Our mission in garnering greater support for

bacterial infection initiated oncogenesis in the

human host, is to eliminate the notion that

only viral infections can serve as oncogenic

events in the human host ( i.e. Human Papilloma virus

[ HPV ] types 16, and 18, Hepatitis C viral subtypes,

Epstein Barr viral infections and Burkitt lymphomas.

Rous Sarcoma virus and malignancies, .. and the list goes

on)




Respectfully,

Alan B. MacDonald, M.D.

Fellow, College of American Pathologists

January 23, 2016

Re: Glioblastoma multiformae 5 cases positive for Burgdorferi borrelia DNA

Posted: Fri 5 Feb 2016 12:16
by inmacdonald
Poster:

F1000 research Poster on Borrelia burgdorferi Glial Infection induced Glioblastomas- A report -Five patients-FISH study of brain biopsies


Link:
https://ogcloud.gonitro.com/p/j9cEWNJ2ECstXKK8dEJ7Qy


Respectfully,

Alan B. MacDonald, MD,FCAP
Feb 5, 2016

Re: Glioblastoma multiformae 5 cases positive for Burgdorferi borrelia DNA

Posted: Tue 24 May 2016 3:22
by ChronicLyme19
inmacdonald wrote:Poster:

F1000 research Poster on Borrelia burgdorferi Glial Infection induced Glioblastomas- A report -Five patients-FISH study of brain biopsies


Link:
https://ogcloud.gonitro.com/p/j9cEWNJ2ECstXKK8dEJ7Qy


Respectfully,

Alan B. MacDonald, MD,FCAP
Feb 5, 2016
Dr. MacDonald, could you please repost the link? This link seems to be broken. I would love to know more about this, I had a friend pass away from glioblastoma.

Thank you so much for all the wonderful work you do <3

Re: Glioblastoma multiformae 5 cases positive for Burgdorferi borrelia DNA

Posted: Sun 28 Apr 2019 18:20
by inmacdonald
Glioblastoma - MacDonald Research - Update - April 28,2019

Link to Original posted Data -

C:\My Web Sites\duray research\durayresearch.wordpress.com\our-work\other-topics\glioblastoma-multiforme-borrelia-dna-in-5-of-5-brain-biopsies\index.html

Update:

I received a telephone communication from the father of a man with biopsy proven
Glioblastoma Multiformae.
This patient's brain biopsy was sent to a reference laboratory with their request
to use molecular biology research methods to evaluate the GBM BIOPSY and Resection tissue for
any and all evidence of Borrelia Species DNa, and for reactivities with
Validated Antibodies for detection of Borrelia burgdorferi protein epitopes.

Positive results in the GBM brain tissue biopsy were returned:
Positive for DNA of The Borrelia Flagellin Gene DNA (Open Reading Frame gene locus) ( Floruescence In situ Hybridization- FISH method)
Positive results for Borrelia specific Proteins in Tumor tissue. (IgM borrelia antibodies bind to tumor)
Microscopy :Flagellae visualized in the midst of Tumor tissue
Blood : Reactive : Borrelia Specific Antibody Detection Method

The patient's Oncologists have continued the usual Chemo-therapies and Proton Beam Irradiation,
but have permitted the patient to receive ORAL Antibiotic combination therapy too, APPROPRIATE FOR
TREATMENT OF RESISTANT BORRELIA INFECTION, Per Infectious Disease Consult.
Preliminary results :
The tumor has been successfully debulked in the parietal lobe of the brain.( Two surgeries: Biopsy, and Excisions)
Radiation therapy ( Proton Beam) has been administered, per GBM Rx Protocols
ChemoTherapy ( ChemoRx)
Depatux ( experimental chemo; a Drug/Antibody Conjugate) ;
Avastin ( Angiogenesis inhibitor)
Keytruda ( Monoclonal immunologic Oncology )
No Cytotoxic Agents to date
Chemo-Rx was suspended because of Therapy related Thrombocytopenia.
which normalized as expected

Post Irradiation imaging of the Brain Surgical site
disclosed pronounced" inflammation patterns"
at the previous tumor site
This was interpreted by Radiology as "" unusual" ,
But.......
Not completely typical for the Brain imaging ---which would be expected from
Chemo-Rx and Radiation Rx effects Alone.

This story is not a conclusive statement of the effectiveness of:
1. Surgery alone ( debulking of Tumor)
2, Chemotherapy alone '( Immunologics but none of the Cytotoxics)
3. Radiation Therapy Alone
4. Possible amelioration of 1.,2., 3. above in this patient, but
Concurrent Antimicrobial Therapy ( oral route)

The Family is investigating the possible Neurosurgical research
placement of a catheter into the lateral ventricle ( on the side of the Tumor) (OMMAYA Reservoir)
which might allow for intraventricular administration of All Oncologist Ordered Antitumor agents
and possibly for Intraventricular adminstration of Combination Antibiotics

---------------------------------------

This morning, I received a telephone call from a SECOND GBM patient.

He has no conclusive Previous Diagnosis of any Lyme like condition.
He does describe excess of exposure to ticks in the past.
He is a Hunter.


He also describes that several of his Hunting Colleagues have died from
GLIOBLASTOMA MULIFORMAE.

i will explore this clinical information further when I interview this Second GBM patient later today..

Respectfully Submitted:

Alan B. MacDonald, MD,
Naples , Florida
April 29,2019

Re: Glioblastoma multiformae 5 cases positive for Burgdorferi borrelia DNA

Posted: Sun 28 Apr 2019 18:22
by inmacdonald
glioblastoma-tumor-binds-borrelia-dna-1a050.png
glioblastoma-tumor-binds-borrelia-dna-1a050.png (179.1 KiB) Viewed 1071 times

Re: Glioblastoma multiformae 5 cases positive for Burgdorferi borrelia DNA

Posted: Mon 29 Apr 2019 13:57
by inmacdonald
Borrelia biofilm - for Comparison
In Human brain from autopsy Neuroborreliosis
Patient
D5458321-AB33-4A23-8C23-8F4EBDF25831.jpeg
D5458321-AB33-4A23-8C23-8F4EBDF25831.jpeg (65.96 KiB) Viewed 1003 times