DNA hybridization BY FISH FINDS BORRELIA IN SPINAL FLUID

General or non-medical topics with information and discussion related to Lyme disease and other tick-borne diseases.
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inmacdonald
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DNA hybridization BY FISH FINDS BORRELIA IN SPINAL FLUID

Post by inmacdonald » Wed 28 Jan 2015 14:39

Molecular Beacon DNA probes for two open reading frames in the Borrelia burgdorferi chromosome have bee developed and Validated for their specificity in hybridizing uniquely to burgdorferi Borrelia DNA.
RESULTS OF VALIDATION STUDIES:
Borrelia burgdorferi pure culture strain ATCC 35210 = POSITIVE
Borrelia Hermsii ,pure culture = NEGATIVE
Also Negative results with ALL. OF. THE FOLLOWING. : Normal human blood, normal by oral squamous epithelial cells,
oral bacterial microbes, E.coli, Candida albicans

Cerebrospinal fluid submitted from a volunteer for molecular evaluation for possible Borrelia DNA IN THE SPINAL FLUID.
( Hospital testing of the spinal fluid produced Normal glucose, Normal protein, normal white blood cell and red blood cell counts,No bacteria by culture of spinal fluid, no spirochetes seen in cytology examination


MolecularBeacon DNA PROBE RESULTS : Positive for Borrelia burgdorferi spirochetes
(See Link to image files). Files stored on server because of vey large file size, which exceeds
The allowed space for postings on this site
link :
https://cloud.gonitro.com/p/c3E6l8iDP5Jl1c0dUcG3Bu

Conclusions:
KENTUCKY resident, adult male, chronically ill, exact cause of chronic illness not diagnosed after multiple physician visits
Lyme ELISA : NEGATIVE
Lyme WESTERN BLOT : NEGATIVE ( only 41 KD band present)
Patient gives a history of multiple tick bites in Kentucky, no erythema Migrans rash noted ay any time


Summation: DNA PROBES (2) demonstrate. Many Borrelia burgdorferi in the cerebrospinal fluid by
FISH method ( Fluorescence In Situ Hybridization) . Many spirochetes seen in fresh up concentrated spinal fluid
By FISH, indicating a very heavy load of infection.
No host immune or leukocytic , macrophage response to the spinal fluid BorrelIa burgdorferi spirochetes.
Chronic illness - un-categorized with conventional medical testing -
Diagnosis: Chronic Lyme Borreliosis, co- infection status not yet addressed by additional proper testing

Respectfully,
Alan B. MacDonald , M.D. , Fellow ,College of American Pathologists
Jan 28, 2015
Last edited by inmacdonald on Sun 1 Feb 2015 13:54, edited 3 times in total.

velvetmagnetta
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Re: DNA hybridization BY FISH FINDS BORRELIA IN SPINAL FLUID

Post by velvetmagnetta » Wed 28 Jan 2015 20:39

This is amazing work. Thank you, Dr. MacDonald. I am so relieved and grateful that you are working on this!

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inmacdonald
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Re: DNA hybridization BY FISH FINDS BORRELIA IN SPINAL FLUID

Post by inmacdonald » Wed 28 Jan 2015 23:43

Dear velvetmagentta and all on LNE,

The FISH DNA hybridization. Will settle the heretofore contentious debates about the REALITY OF
CHRONIC BORRELIA ILLNESSES.

I WILL PROVIDE THE METHODOLOGY AND THE DETAILS OF MANUFACTURE OF THE
MOLECULAR beacon DNA PROBES IN AN UPCOMING PEER REVIEWED PUBLICATION.

AN Epifluorescent microscope is required to accomplish the microscopic exams.

For a limited time I will continue to receive blood smears and Spinal fluid specimens
To extend these observations.

I will donate the peer reviewed methodology Free of charge to persons who
Own an Epifluorescent microscope.


The Inconvient Truth of the reality of Seronegative Lyme Borreliosis or IgM reactive serology in
Patients with " Late " chronic Borreliosis. Will be embraced as multiple scientists in international
Laboratories. Implement the FISH METHOD. for Borrelia detection in blood, seminal fluid, spinal fluid,amniotic fluid,
Arteritis
Or...chamber or the eye Aqueous ...
and biopsies from solid organs or examination of autopsy tissues, with the FISH METHOD USING BORRELIA BURGDORFERI

DNA hybridization will settle the contentious category of chronic Borreliosis,
Once and for all time...

Links to additional FISH POSITIVE RESULTS FROM CHRONIC BORRELIOSIS AND FROM AUTOPSY CHRONIC BORRELIOSIS:


Respectfully

Alan B. MacDonald, MD,FCAP
JANUARY 28, 2015
Last edited by inmacdonald on Fri 30 Jan 2015 11:33, edited 1 time in total.

velvetmagnetta
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Re: DNA hybridization BY FISH FINDS BORRELIA IN SPINAL FLUID

Post by velvetmagnetta » Thu 29 Jan 2015 3:52

I know you work closely with medical professionals and researchers in Europe - will a FISH method be able to detect the B. garinii species as some point as well? I live in America and suspect I may have that species along with many people on both the East and West US coasts.

This Lyme species is supposed to be "rare" in the united states, so doctors here seem to think they don't have to know anything about neurological Lyme disease symptoms, treatment, and aftermath of infection.

This is very frustrating to me not only because my neurological problems are severe but also because...well...it is just an asinine thing to say that European ticks can't make their way into the US on migrating birds or, especially, on humans and pets in this day and age of modern airplane travel.

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inmacdonald
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Re: DNA hybridization BY FISH FINDS BORRELIA IN SPINAL FLUID

Post by inmacdonald » Thu 29 Jan 2015 13:26

The technique will pick up
EUROPEAN Borrelia burgdorferi and will pick up Borrelia Afzelii and Borrelia Garinii too

Best
Alan

duncan
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Re: DNA hybridization BY FISH FINDS BORRELIA IN SPINAL FLUID

Post by duncan » Thu 29 Jan 2015 13:29

Is this a relatively new technique? Is it likely to be challenged? Will agencies like the NIH embrace it?

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inmacdonald
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Re: DNA hybridization BY FISH FINDS BORRELIA IN SPINAL FLUID

Post by inmacdonald » Thu 29 Jan 2015 16:24

Google:

Choose search terms ." Borrelia and FISH"
You will find the current bibliography...

Then Google
Choose search terms : " FISH AND SPIROCHETES"
You will find a bibliography....


DNA probes are used commonly in Pathology and in Microbiology.....

In Situ Hybridization. ( ISH ) is standardized!.
Entire textbooks are published !!!
ISH
AND
FISH
DO AN AMAZON BOOKS SEARCH TO VERIFY.!!


THE TECHNIQUE OF in Situ Hybridization has been around in research for Decades.

It works
It makes hybridized target VISIBLE UNDER THE MICROSCOPE.


THE FDA HAS NO JURISDICTION OVER in situ hybridization. Or FISH either

Alan B. MacDonald, MD, FCAP
JANUARY 29, 2015

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ChronicLyme19
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Location: NY, USA

Re: DNA hybridization BY FISH FINDS BORRELIA IN SPINAL FLUID

Post by ChronicLyme19 » Fri 30 Jan 2015 18:29

inmacdonald wrote:I WILL PROVIDE THE METHODOLOGY AND THE DETAILS OF MANUFACTURE OF THE
MOLECULAR beacon DNA PROBES IN AN UPCOMING PEER REVIEWED PUBLICATION.
:mrgreen: I look forward to reading it.
Half of what you are taught is incorrect, but which half? What if there's another half missing?

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inmacdonald
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Re: DNA hybridization BY FISH FINDS BORRELIA IN SPINAL FLUID

Post by inmacdonald » Sun 15 Feb 2015 17:48

Image Compendium of borrelia spirochetes in Human Cerebrospinal Fluid -Kentucky Adult

FISH method using Molecular Beacon DNa Probe BBO 0147

Link:

https://cloud.gonitro.com/p/kfDKApzZTQ-LTWczKh1DYX


Respectfully Submitted,

Alan B. MacDonald, MD, FCAP
February 15,2015

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inmacdonald
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Re: DNA hybridization BY FISH FINDS BORRELIA IN SPINAL FLUID

Post by inmacdonald » Tue 3 Mar 2015 16:54

FOR THOSE WITH AN INTEREST IN THE DIAGNOSTIC POWER OF

FISH METHOD (Fluorescent In Situ Dna/mRNA Hybridization)

for the detection of Borrelia burgdorferi species and more recently for
detection of Borrelia Miyamotoi sl group

in human Blood

and in Human Autopsy Brain tissues

Please see the "MEDICAL" section of this forum

The Exact DNA nucleotide Sequence for the FISH Flagelin B (FlaB) Dna Probe is as follows:

TGG Gag TTT CTG GTA AGA TTAA

( lower case is correction of originally posted TYPO)

Respectfully,
Alan B.MacDonald, MD,Fellow,College of American Pathologists
May 3,2015
Last edited by inmacdonald on Mon 9 Mar 2015 15:40, edited 1 time in total.

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