Information about microscopy (for the member who asked)

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Re: Information about microscopy (for the member who asked)

Post by X-member » Thu 20 Dec 2012 12:07

From Bagges post (earlier):
Science-Based Medicine offers evidenced-based information about microscopy ....
A manual from CDC:

"DARKFIELD MICROSCOPY FOR THE DETECTION AND IDENTIFICATION OF TREPONEMA PALLIDUM"

http://www.cdc.gov/std/syphilis/manual-1998/CHAPT5.pdf

A quote:
Darkfield Microscopy
3,4
The standard brightfield microscope may be equipped for darkfield examination by replacing the
brightfield, or Abbe, condenser with either a double- or single-reflecting darkfield condenser.
Illumination for darkfield microscopy is obtained when light rays strike the object in the field at an
oblique angle so that no direct light rays enter the microscope objective, only the rays reflected
from the object. Therefore, the object appears self-luminous against a dark background. When a
fluid containing particles, including bacteria or treponemes, is placed on a slide, the oblique rays
are reflected from the surfaces upward into the barrel of the microscope; these particles appear
brightly illuminated against a black background. This type of illumination can be obtained by using
a double-reflecting darkfield condenser (Fig 4:1) or a single-reflecting darkfield condenser (Fig
4:2). In the double-reflecting darkfield condenser, two reflecting surfaces produce intense
illumination; however, this type of condenser requires precise focusing and accurate centering.

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Re: Information about microscopy (for the member who asked)

Post by X-member » Mon 19 Aug 2013 21:47

The Global Search for Education: Norway - Ticks

http://www.huffingtonpost.com/c-m-rubin ... 77332.html

A quote:
Drs. Mysterud and Laane - How does your approach to testing and findings differ from the published blood analysis research that has been done for Borrelia to date?

Our method differs from others in its simplicity and low costs. The needs for fast and simple methods are overwhelming, as DNA or immune methods, unfortunately, meet many unexpected technical problems, and often fail.

Our method is based upon live blood microscopy. Bacteria become visible by optical changes involving refraction index, swelling and spreading of cells, which result in inflated structures well above the optical resolution capacities for a light microscope. Phase-contrast microscopy is used. The main trick is spreading the red blood cells apart in an anoxic salt solution so that even very thin bacteria become visible against the diluted blood plasma. The method is useful not only for spirochetes, but also other bacteria, apicomplex parasites, microfilaria, fungi, etc.

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Re: Information about microscopy (for the member who asked)

Post by X-member » Sun 9 Mar 2014 3:09

Borrelia Spirochete Morphology in Peripheral Blood

http://counsellingme.com/microscopy/Exp ... gfont.html

http://counsellingme.com/microscopy/Spi ... ology.html

Morphology, culture and more.

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Re: Information about microscopy (for the member who asked)

Post by X-member » Mon 1 Sep 2014 1:36

Intracellular Borrelia Spirochaetes Reproduce Inside Erythrocytes

http://counsellingme.com/microscopy/int ... hetes.html

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Re: Information about microscopy (for the member who asked)

Post by X-member » Wed 29 Apr 2015 14:41

Demonstrating the Intracellularity of Borrelia burgdorferi

http://www.lymenet.de/literatur/intracel.htm

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Re: Information about microscopy (for the member who asked)

Post by X-member » Wed 29 Apr 2015 14:45

Babesiosis (Wikipedia)

http://en.wikipedia.org/wiki/Babesiosis

A quote:
Only specialized laboratories can adequately diagnose Babesia infection in humans, and as a result, Babesia infections are considered highly under-reported. It develops in patients who live in or travel to an endemic area or receive a contaminated blood transfusion within the preceding 9 weeks, so this aspect of the medical history is vital.[6] Babesiosis may be suspected when a person with such an exposure history develops persistent fevers and hemolytic anemia. The definitive diagnostic test is the identification of parasites on a Giemsa-stained thin blood smear.[6]

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Re: Information about microscopy (for the member who asked)

Post by X-member » Sat 28 Nov 2015 9:18

Perhaps some people that understand Norwegian need some help when it comes to microscopy?

Moderne mikroskopi med enkle metoder
av Morten Motzfeldt Laane, Thore Lie

HÄFTAD , Norska (bokmål), 2007


http://www.adlibris.com/se/bok/moderne- ... 8274772816
Mikroskopet er et av de viktigste forskningsinstrumentene innen blant annet biologi og medisin. De ulike mikroskopiske metodene har stor betydning innen fagfelter som biologisk systematikk, medisinsk diagnostikk, sykdomsbekjempelse, genteknologi, næringsmiddelkontroll, materialsikkerhet, og etterforskningsteknikk. I denne boken er det lagt vekt på å beskrive moderne mikroskopi på en lettfattelig måte, slik at mikroskopet fungerer etter forutsetningene og brukeren kommer frem til et vellykket resultat. På tross av mikroskopiens store betydning, har faget vært ansett som omkostningskrevende og teknisk vanskelig. Her viser forfatterne hvordan man med enkle digitale teknikker og rimelige omkostninger kan oppnå resultater som er fullt på høyde med dem man får fra avanserte forskningsmikroskop. Boken inneholder også beskrivelser av de vanligste fargemetodene for fremstilling av preparater og gir en oversikt over en rekke mikroskopiske teknikker, både enkle og mer avanserte. Boken gir nyttige grunnkunnskaper for alle som bruker mikroskopet, både yrkesmessig og på hobbybasis. Morten Motzfeldt Laane, dr. philos. og professor ved Institutt for molekylær biovitenskap, Universitetet i Oslo, der han arbeider med cellebiologiske og biofysiske problemstillinger. Han har utviklet en rekke teknikker innen både lys- og elektronmikroskopi. Fellow of Royal Microscopical Society, Oxford. Thore Lie, dr.scient., Ph.D. og forlagsredaktør. Han har tidligere arbeidet som forskningsstipendiat (NAVF) ved Universitetet i Oslo og har omfattende forsknings- og undervisningserfaring innen mikroskopi. Fellow of The Linnean Society, London.

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Re: Information about microscopy (for the member who asked)

Post by X-member » Fri 1 Jan 2016 22:38

Borrelia and Microscopy

http://counsellingme.com/microscopy/How ... Intro.html

A quote:
The aim of these pages is to be a guide on how to set-up a microscope capable of observing spirochetes. It is intended for beginners, or intermediates who have not used darkfield and/or maximum magnification set-ups.

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Re: Information about microscopy (for the member who asked)

Post by X-member » Tue 2 Feb 2016 4:53

From Sweden:

A STING from a Tick:
Epidemiology, Ecology and Clinical Aspects of Lyme Borreliosis (2014)


Peter Wilhelmsson, Linköping University


Dissertation

http://liu.diva-portal.org/smash/get/di ... TEXT01.pdf

A quote:
Microscopic visualization of live Borrelia spirochetes offers the strongest of all proofs that an infection is present. Borrelia burgdorferi can be visualized directly in infected vectors, reservoir hosts, laboratory animals and clinical specimens from patients with LB using dark-field or phase-contrast microscopy.

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Re: Information about microscopy (for the member who asked)

Post by X-member » Tue 16 Feb 2016 12:59

32 of 40 chronically ill have spirochetes in their blood (blog)

https://newsaboutdisease.wordpress.com/ ... eir-blood/

If you have any questions about this do not ask me, I'm not involved at all in this.

Edit to add:

A translated quote (from the Swedish text):
"Lyme Wars" - "Chronic Borreliosis" - "Residual Symptoms" mentioned frequently
There is no patient who wants war. Anyone who is sick wants to be healthy. A stronger motivation is hardly needed. Some believe that "chronic borreliosis" also includes residual symptoms and others argue that chronic borreliosis actually only consists of few criteria that the Public Health Agency set up.
Chronic borreliosis stands for late Lyme disease (stage 3) in Sweden.

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