Persistent Borrelia Infection in Patients with Ongoing Symptoms of Lyme Disease

Topics with information and discussion about published studies related to Lyme disease and other tick-borne diseases.

Psilociraptor
Posts: 20
Joined: Fri 29 Apr 2016 15:57

Re: Persistent Borrelia Infection in Patients with Ongoing Symptoms of Lyme Disease

Postby Psilociraptor » Fri 6 Jul 2018 15:47

So... given that post treatment culture failure is half the reason this debate spawned... what exactly did they do differently to make it work here? Seems like they didn't talk much about what they attributed their success to.

duncan
Posts: 1365
Joined: Wed 5 Sep 2012 18:48

Re: Persistent Borrelia Infection in Patients with Ongoing Symptoms of Lyme Disease

Postby duncan » Fri 6 Jul 2018 15:57

I suspect part of the answer lies in testing tissue.

Martian
Posts: 1944
Joined: Thu 26 Jul 2007 18:29
Location: Friesland, the Netherlands

Re: Persistent Borrelia Infection in Patients with Ongoing Symptoms of Lyme Disease

Postby Martian » Sat 7 Jul 2018 11:04

Abstract of the publication:

Healthcare (Basel). 2018 Apr 14;6(2). pii: E33. doi: 10.3390/healthcare6020033.

Persistent Borrelia Infection in Patients with Ongoing Symptoms of Lyme Disease.

Middelveen MJ, Sapi E, Burke J, Filush KR, Franco A, Fesler MC, Stricker RB.

Abstract

INTRODUCTION: Lyme disease is a tickborne illness that generates controversy among medical providers and researchers. One of the key topics of debate is the existence of persistent infection with the Lyme spirochete, Borreliaburgdorferi, in patients who have been treated with recommended doses of antibiotics yet remain symptomatic. Persistent spirochetal infection despite antibiotic therapy has recently been demonstrated in non-human primates. We present evidence of persistent Borrelia infection despite antibiotic therapy in patients with ongoing Lyme disease symptoms.

METHODS: In this pilot study, culture of body fluids and tissues was performed in a randomly selected group of 12 patients with persistent Lyme disease symptoms who had been treated or who were being treated with antibiotics. Cultures were also performed on a group of ten control subjects without Lyme disease. The cultures were subjected to corroborative microscopic, histopathological and molecular testing for Borrelia organisms in four independent laboratories in a blinded manner.

RESULTS: Motile spirochetes identified histopathologically as Borrelia were detected in culture specimens, and these spirochetes were genetically identified as Borreliaburgdorferi by three distinct polymerase chain reaction (PCR)-based approaches. Spirochetes identified as Borrelia burgdorferi were cultured from the blood of seven subjects, from the genital secretions of ten subjects, and from a skin lesion of one subject. Cultures from control subjects without Lyme disease were negative for Borrelia using these methods.

CONCLUSIONS: Using multiple corroborative detection methods, we showed that patients with persistent Lyme disease symptoms may have ongoing spirochetal infection despite antibiotic treatment, similar to findings in non-human primates. The optimal treatment for persistent Borrelia infection remains to be determined.

KEYWORDS: Borrelia burgdorferi; Lyme disease; chronic infection; spirochete culture; tickborne disease

PMID: 29662016 PMCID: PMC6023324 DOI: 10.3390/healthcare6020033


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Psilociraptor
Posts: 20
Joined: Fri 29 Apr 2016 15:57

Re: Persistent Borrelia Infection in Patients with Ongoing Symptoms of Lyme Disease

Postby Psilociraptor » Wed 11 Jul 2018 2:41

duncan wrote:I suspect part of the answer lies in testing tissue.


Maybe I missed something, but wasn't it mostly blood and genital secretions? Either way it seems worthy of a discussion since it will obviously be a point of contention for critics.


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